Psoriasis und Nicotinsäure
Try the new Google Patents, with machine-classified Google Scholar results, and Japanese and South Korean patents. Zubereitungen von mehreren antichlamydialen mitteln zur diagnose und behandlung von durch chlamydia verursachten Psoriasis und Nicotinsäure Preparations of several antichlamydialen submit to diagnose and treat infections caused by chlamydia translated from German DE T2. The composition of claim 1, wherein one of the means selected from the group consisting of macrolide antibiotics and azalide antibiotics.
The composition of claim 1, ICD-Code Pruritus the effective against the elementary Psoriasis und Nicotinsäure phase is a reducing agent disulfide agent.
Zusammensetzung nach Anspruch 4, worin das reduzierende Disulfid-Mittel Penicillamin ist. The composition of claim 4, wherein said disulfide reducing agent is penicillamine. Zusammensetzung nach Anspruch 1, worin das gegen die kryptische Phase wirksame Mittel eine aromatische Psoriasis und Nicotinsäure ist.
The composition of claim 1, wherein the effective against the cryptic phase agent is an aromatic nitro compound. The composition of claim 7, wherein the aromatic nitro compound is selected from the group consisting of nitroimidazoles, nitrofurans, more info, derivatives and combinations thereof.
Use of a pharmaceutical composition according to any one of claims for the manufacture of a medicament for treatment of a chlamydial infection. An ex vivo method to clear the biological material of Chlamydia, comprising the step of contacting the biological material with at more info two agents, each of which is against a different phase of chlamydial life cycle is effective, wherein one of the agents is a rifamycin until the biological material is negative for Chlamydia according to a test Psoriasis und Nicotinsäure which the elementary body phase Chlamydia, replicating phase Chlamydia, and cryptic phase Chlamydia.
An ex vivo method of claim 11, wherein the effective against the elementary body phase is a reducing agent disulfide agent. Ex vivo Verfahren nach Anspruch 13, wobei das reduzierende Disulfid-Mittel Penicillamin ist. An ex vivo method of claim 13, wherein said disulfide reducing agent is penicillamine. Ex vivo Verfahren nach Anspruch 10, wobei das gegen die kryptische Phase wirksame Mittel eine aromatische Nitroverbindung ist. An ex vivo method of claim 10, wherein the effective against the cryptic phase agent is an aromatic nitro compound.
An ex vivo method of claim 16, wherein the aromatic nitro compound is selected from the group consisting of nitroimidazoles, nitrofurans, analogs, derivatives and combinations thereof.
An ex vivo method of claim 10, wherein one of the means selected from the group consisting of macrolide antibiotics and azalide antibiotics. An ex vivo method of claim 10, wherein the test, with the elementary body phase Chlamydia, replicating phase Chlamydia, and cryptic phase of chlamydial is detected, comprises a nucleic acid amplification step. Ex vivo Verfahren nach Anspruch 19, wobei der Test die Schritte umfasst: Use of at least two agents, each of which is against a different phase of chlamydial life Psoriasis und Nicotinsäure is effective, wirksames Mittel gegen Schuppenflechte one of the agents is a rifamycin in the manufacture of a medicament or medicaments Psoriasis und Nicotinsäure simultaneous administration, for clearing biological material of Chlamydia until the biological material is negative for Psoriasis und Nicotinsäure according to a test by which the elementary body phase Chlamydia, replicating phase Chlamydia, and cryptic phase Chlamydia is detected.
Use according to claim 22, wherein the effective against the elementary body phase is a reducing agent disulfide reagent. Verwendung nach Anspruch 24, wobei das reduzierende Disulfid-Mittel Penicillamin ist. Use according to claim 24, wherein said Psoriasis und Nicotinsäure reducing agent is Psoriasis und Nicotinsäure. Verwendung nach Anspruch 21, wobei das gegen die kryptische Phase wirksame Mittel eine aromatische Nitroverbindung ist.
Use according to claim 21, wherein the effective against the cryptic phase agent is an aromatic nitro compound. Use Psoriasis und Nicotinsäure to claim 27, wherein the aromatic nitro compound is selected from the group consisting of nitroimidazoles, nitrofurans, analogs, derivatives and combinations thereof.
Use according to claim 21, wherein Psoriasis und Nicotinsäure of the means selected from the group consisting of macrolide antibiotics and azalide antibiotics. Use according to claim 21, wherein the test, with the elementary body phase Chlamydia, replicating phase Psoriasis und Nicotinsäure, and cryptic phase of chlamydial is detected, comprises a nucleic acid amplification step.
Verwendung nach Anspruch 30, average Kopfhaut-Psoriasis-Pillen Hautpflege der Test die Schritte umfasst: Chlamydiae are obligate intracellular microorganisms Psoriasis und Nicotinsäure parasitize in eukaryotic cells and ubiquitous occur in the animal kingdom.
Die Mitglieder der Gattung von Chlamydia werden als Bakterien mit einem einzigartigen biphasischen Entwicklungszyklus angesehen, der unterschiedliche morphologische und Krank mit viele Psoriasis, Tage wie Formen aufweist. The members of the genus Chlamydia are Psoriasis und Nicotinsäure to be bacteria with a unique biphasic life cycle, having different morphological and functional forms.
This development proper growth cycle alternates between 1 intracellular life forms, two of which are currently known, a metabolically active, replicating organism, known as the reticular bodies Psoriasis und Nicotinsäure and a persistent, non-replicating organism, known as cryptic phase and 2 an extracellular form of life that metabolically inactive is Psoriasis und Nicotinsäure infectious, shape, known as the elementary or elementary bodies EB.
EBs are small nminfectious spores similar shapes that are not replicating metabolically Psoriasis und Nicotinsäure and are frequently found in the acellular milieu. Angriffen wie beispielsweise Enzymabbau, Ultraschallbehandlung und osmotischem Druck resistent. EBs are resistant to various physical insults or attacks such as enzyme digestion, sonication and osmotic pressure.
It is believed that these physical stability of a result of the extensive Disulfidvernetzung of the cysteine-rich main outer membrane protein MOMP Bavoil et al, Infection and Immunity.
Under oxidizing conditions in the acellular milieu of the host outer membrane of EBs is both relatively impermeable and resistant to inactivation.
EBs are therefore well suited for long enough Psoriasis und Nicotinsäure survive outside of those hosts to a new host in the form of condensation droplets core or core Theunissen et just click for source, Applied Environmental Microbiology, Infections caused by members of the genus Chlamydiae induce cellular level a significant inflammatory response. Gender-tissue injuries that were generated for example by Chlamydia trachomatis, often evoke a strong influx of lymphocytes, macrophages, and plasma cells, suggesting the development of a humoral and cellular immunity.
However, the initial infection can often Psoriasis und Nicotinsäure out different and even asymptomatic symptomatology. As soon as the Chlamydia have completely fixed and established, they are often difficult to eradicate with Psoriasis und Nicotinsäure relapse after antibiotic therapy. Notes also show that Chlamydia can go into Psoriasis und Nicotinsäure and then repelled in amounts that are too small to be reliably detected by a culture.
Chlamydia pneumoniae hier "C. Chlamydia pneumoniae here "C. This newly recognized Psoriasis und Nicotinsäure usually infects the upper and lower respiratory tract, which was now found that it Psoriasis und Nicotinsäure everywhere in humans. Es ist so daß in psoriasis, dass C. Baun AL, eg Microbiology of Chlamydia, Boca Raton, FL, CRC Press,pp.
It Masken Psoriasis known that C. AL Baun, eg Microbiology of Chlamydia, Boca Raton, FL, CRC Press,pp. Trotz dieser Darstellung einer Empfindlichkeit in vitro kann die Infektion mit C.
Despite this representation of in vitro susceptibility to the infection can lead to the following with C. In Psoriasis und Nicotinsäure studies on the persistence of Chlamydia in spite of a specific and appropriate antibiotic therapy, have suggested that the presence of antibiotics, the formation of an intracellular, non-replicating state Beatty et al, Microbiol Rev.
Entfernung des belastenden Einflusses gestattet dem Organismus die Replikation wieder aufzunehmen. Removal of the harmful influence allows the organism resume replication again.
Thus, the organism in this manner, the current antibiotic therapy used in clinical practice, to escape. In view of the chronic and persistent nature of the infection with Chlamydia, there is a need for reliable, accurate method for the diagnosis of a pathogenic infection, as well as of therapeutic approaches Shampoo hilft bei Psoriasis handle the infection.
Due to the highly infectious nature of the EBs of chlamydia and their ability to infect cells again, there is also a need for an anti-chlamydial therapy that completely eradicates this pathogen, thus improving long-term sequelae of such chronic infections be prevented. The present invention provides a unique Psoriasis und Nicotinsäure for the diagnosis and handling of infection with Chlamydia species, particularly C.
Accordingly, one embodiment of the invention the use of a combination of anti-chlamydial agents consisting of at least two agents, each of which is directed of which against a different phase of chlamydial life cycle, wherein one of the agents in Psoriasis und Nicotinsäure manufacture of a medicament for treating a infection by Chlamydia species is rifamycin.
For example, the agents may be selected from the following groups: Das Chlamydien-Pathogen kann schneller beseitigt werden, wenn eine Kombination verabreicht wird, die Mittel umfasst, die gegen jede Phase des Lebenszyklus der Der Psoriasis bei Kindern gerichtet sind. The Chlamydia pathogen can be removed faster when a combination is administered, comprising means, which are directed against each phase of the life cycle of chlamydia.
The invention also relates to novel combinations of anti-chlamydial agents and new Psoriasis und Nicotinsäure compositions comprising at least two anti-chlamydial agents, each of which is directed of which against a different phase of the life cycle of chlamydia, wherein one of the means rifamycin and wherein the agents are selected from the group consisting of: These compositions and combinations of agents may further Psoriasis und Nicotinsäure one or a combination Psoriasis und Nicotinsäure additional compounds, anti-inflammatory agents, the immune suppressive agents and anti-Prophyrin- or prophyriale means include.
The use of the combination of anti-chlamydial agents or compositions thereof, for the manufacture of a medicament for the management of an infection with Chlamydia is also described. In a particular embodiment, the means may be arranged individually mixed or arranged according to label instructions.
The invention provides the basis for a new treatment available that comprises a specific directed against the phase of the elementary of the life cycle of Chlamydia means that if it is applied for a sufficient length of time, allows the active infection without the generation of infectious EBs can be terminated. In order to promote patient compliance during a therapy course, the invention provides a base for the packaging, Psoriasis und Nicotinsäure agents described Psoriasis und Nicotinsäure for handling a chlamydia infection, ready.
Beispielsweise kann eine Packung mindestens zwei unterschiedliche Mittel umfassen, wobei jedes von denen gegen eine unterschiedliche Phase des Lebenszyklus der Chlamydien gerichtet ist, worin eines der Mittel Rifamycin ist. For example, a package comprising at least two different means, each of which is directed against a different phase of chlamydial life cycle, wherein one of the means is rifamycin.
These agents may be selected from the group consisting of: Alternatively, as mentioned above, additional compounds equally be present in the package. Eine bevorzugte Packung wird mehrere Mittel umfassen, die Psoriasis und Nicotinsäure zwei, jedoch vorzugsweise, gegen alle die Stadien des Lebenszyklus der Chlamydien gerichtet sind.
A preferred pack will Psoriasis und Nicotinsäure one or more agents which are directed against two, but preferably against all the stages of the life cycle of chlamydia. Die Packung kann eine Einheitendosierung der Mittel bereitstellen oder kann mehrere Einheitendosierungen umfassen, und kann mit Information, wie beispielsweise, dem Modus und der Psoriasis und Nicotinsäure beispielsweise getrennt, gleichzeitig oder sequentiell von jeder darin beinhalteten Komponente gekennzeichnet sein.
The package can provide a unit dosage of the agent or may visit web page a plurality of unit dosages, and may with information, for example, the mode of administration and instruction for example, separately, simultaneously or sequentially of each component contained therein be Psoriasis und Nicotinsäure. The method includes quantifying the antibody titer or another measure or detection against the pathogen and comparing the measure with the antibody-detection, which was quantified at an earlier time in therapy, wherein the difference between the detections of the course of the Therapy displays.
The invention also provides the basis for a method for monitoring the course of therapy for treating an infection caused by Chlamydia, comprising determining the presence or absence of Chlamydia in an infected individual in time intervals during the course of therapy. In a particular embodiment, this is determined by a PCR test for pathogen DNA or an antigen capture assay for the pathogen.
Eine Detektion der Anwesenheit von Chlamydien in einer Probe biologischen Materials, das von einem Individuum entnommen wurde, von dem angenommen wurde, dass es damit infiziert ist, ist bei der Bestimmung des Therapieverlaufs und der zu verwendenden Mittel entscheidend. A detection of the presence of Chlamydia in a Psoriasis und Nicotinsäure of biological material that has been taken from an individual from which article source was assumed that it is infected therewith, is crucial in determining the course of therapy and the appropriate agents.
Dies kann durch Detektieren der Anwesenheit von DNA erreicht werden, die MOMP von Chlamydia oder von anderen Genen von Chlamydien in dem Individuum kodieren.
This can be achieved by detecting the presence of DNA encoding the MOMP of Chlamydia or of other genes of Chlamydia in the individual. Diseases which are associated with a chlamydial infection, such as inflammatory diseases, autoimmune diseases and diseases in which the individual has a weakened immune system, can handling i.
Preferably, the presence or absence of a signal for the amplified DNA of Chlamydia MOMP or other Chlamydia a protein coding DNA is determined. The learn more here of a signal shows a decrease with the degree of infection, which is detected by an amplification of nucleic acid Punkt bei einer für puts eradication of the microorganism near strong.
The sensitivity tests described herein are particularly useful as drug-search tool for assessing the activity of single agents or combinations of agents against infection with Chlamydia. In particular embodiments, the chlamydial species C. Further, by an amplification of the nucleic acid e. PCRa method for identifying cells is described here which include a cryptic form a Chlamydia species, which check this out subjecting cultured cells to a protease digestion, stopping the protease activity, exposing Sie Psoriasis Club FREE cells to a suitable heat-stable DNA polymerase, dNTPs and labeled primers e.
A method of identifying cells that contain a cryptic form of Chlamydia comprises, treating of cultured cells is believed by those that they are infected with chlamydia, reducing with a disulfide agent, subjecting the cultured cells to a protease digestion, exposing the cells a suitable polymerase, dNTPs and primers for DNA amplification of nucleic acid encoding a Chlamydial protein, exposing the cells to a reporter molecule-enzyme, exposing the continue reading to an appropriate substrate for the reporter enzyme, and determining the presence of a cryptic form of chlamydial by visualization of the amplified DNA sequence encoding a Chlamydial Psoriasis und Nicotinsäure. Vorzugsweise Psoriasis und Nicotinsäure das Psoriasis und Nicotinsäure PCR und die Primer sind CHLMOMPDB2 and CHLMOMPCB2 von Chlamydia pneumoniae.
Preferably, the PCR amplification and primer are CHLMOMPDB2 and CHLMOMPCB2 of Chlamydia pneumoniae. A similar method can be used as an assay for identifying an agent which is effective against a cryptic form of chlamydia. Accordingly, the method, treatment of cultured cells that were grown in the absence of cycloheximide, which are believed to be infected with Chlamydia, Chlamydia to replicate with a disulfide reducing agent, allowing to adding comprises a test agentPsoriasis und Nicotinsäure the cultured cells to a protease digestion, exposing the cells to a suitable polymerase, dNTPs and primers Psoriasis und Nicotinsäure DNA amplification of a Chlamydia protein, exposing the cells to a reporter enzyme molecule, exposing the cells to an appropriate substrate for the reporter enzyme, and determining the presence of a cryptic form of Chlamydia by visualization of the amplified DNA encoding a Chlamydial protein, such as MOMP encoded.
Is a method for detecting elementary bodies of Chlamydia in a sample is also disclosed, comprising, contacting said sample with a disulfide reducing agent before a DNA amplification method is used to detect chlamydial DNA in the sample. The present invention relates to ex vivo method for clarifying of biological material that is infected with chlamydia. According to the method, a biological material is released from a chlamydial infection by the Example Zwiebelschalen Psoriasis You material with at least two means, but preferably three means, is brought into contact, each of which is directed against a different phase of the life cycle of chlamydia, wherein one of the medium is rifamycin until the biological material is no longer tested positive for chlamydia.
The agents can be selected from the group consisting of: In one embodiment, the agent which is directed against the elementary body phase is a disulfide reducing agent.
Nitrofurane, Analoge, Derivate und Kombinationen davon. In another embodiment, the agent directed against a cryptic phase is a nitroaromatic compound such as nitroimidazoles, Nitrofluane or nitrofurans, analogs, derivatives Psoriasis und Nicotinsäure combinations thereof.
Click at this page illustrative purposes, such as HeLa CF, HL-CF, HCF, HuEVEC-CF and McCoy CF, the biological material was clarified by an infection with chlamydia, a continuous and stable cell line, it being " CF "a shorthand note for" Chlamydia is free ". Alternatively, the biological material is an animal, Psoriasis und Nicotinsäure as a mouse, rabbit or other animal model learn more here, which is negative for chlamydia.
The invention provides a method for maintaining a basis for chlamydia free state Psoriasis und Nicotinsäure animals and cell lines that have been cleared of infection with Chlamydia by the method of this invention, or Psoriasis und Nicotinsäure never infected as their chlamydia-free progeny or offspring.
In particular, to be administered to a chlamydial free Psoriasis und Nicotinsäure or animals continue reading of nutrients can be treated so that any elementary bodies of Chlamydia thereof are inactivated or removed. This can be achieved in that the nutrients are exposed for a period of continue reading and Psoriasis und Nicotinsäure degree of exposure Psoriasis und Nicotinsäure gamma-irradiation, which is Psoriasis und Nicotinsäure to inactivate the elementary bodies.
In addition, or alternatively, a source of nutrients are carried out through a filtration system to remove the elementary bodies of Chlamydia thereof physically. Optionally, the source of nutrients may be first treated with a disulfide reducing agent, such as dithiothreitol, before the filtration step is performed. The filter should have Psoriasis und Nicotinsäure suitable size so that objects which Psoriasis und Nicotinsäure Propolis-Salbe Psoriasis than 0.
For purposes of illustration herein is a method for detecting viable chlamydia is described in a biological material, which is believed that it is contaminated, comprising, cultivating chlamydia free cells or animals in the presence of biological material and subsequently determining the presence or absence of viable Psoriasis und Nicotinsäure in culture.
Erkrankung bedingt ist, beschrieben. Here, a method of differentiating a porphyria, which is caused by Chlamydia species from a porphyria, which is caused by a genetic disease or faulty control, described merely for illustrative purposes. A method for diagnosing a secondary porphyria, which is caused in a subject by Chlamydia having symptoms associated therewith comprising, determining the presence or amount of the required enzymes in the heme biosynthesis in red blood cells of the individual and a determining the presence of Chlamydia in the individual.
Furthermore, a method for differentiating one caused by Chlamydia secondary porphyria, from Psoriasis und Nicotinsäure which is caused by a genetic dysregulation in an individual, treating the chlamydial infection with numerous stages of their life cycle and then judging whether porphyrins were reduced, with a decrease in the porphyrin indicates that the porphyria is secondary and is caused by chlamydia. For illustrative purposes, a method for treating a porphyria, which is caused by Chlamydia in a subject in need thereof, comprising reducing the levels of active stages, latent stages and elementary of the pathogen from the subject, and administering one or more compounds, the unfavorable reduce effects that are associated with a secondary porphyria.
The method may Psoriasis und Nicotinsäure comprise administering a compound that reduces the adverse effects associated with porphyrins a porphyria. Die Verbindung kann Cimetidin sein. The compound may be cimetidine. This method can also be combined to valuable way with additional steps, including at least one of administering of antioxidants, orally administering active charcoal, administering a regimen with a high carbohydrate diet, administering hydroxychloroquine, administration of benzodiazepine drugs, performing a hemodialysisperforming Psoriasis und Nicotinsäure plasmapheresis and administration of chelating agents and intravenous administration of hematin.
For purposes of illustration elevated porphyrin can be detected in a subject characterized by an individual Psoriasis und Nicotinsäure tested for antibodies to porphyrin, wherein a defect by detecting antibodies to B can be diagnosed. For purposes of illustration, the invention may provide a basis for a process which can be by Psoriasis und Nicotinsäure a computerized Psoriasis und Nicotinsäure, for example, Psoriasis und Nicotinsäure formulate a drug therapy Psoriasis und Nicotinsäure manipulating a disease caused by Chlamydia infection, automated.
Das Verfahren umfasst, Bestimmen von Zielen bzw. The method includes determining objectives or targets within the chlamydial life cycle for each specific target, identifying agents that are active against the target, and combining at least a subset of identified assets in order to handle a disease caused by Chlamydia infection is a combination provide therapy, said means being alone in the subset against different targets in the chlamydial life cycle active. Include the objectives, identifying phases of the life cycle of Chlamydia, wherein for each identified lifecycle phase is at least one more vulnerable and vulnerable aspect of the organism during this life cycle phase, determined, each specific vulnerable aspect of a goal during the life cycle of Chlamydia shall determine.
Agents which have been identified by the method are subsequently tested using the described herein susceptibility test procedure, wherein Psoriasis und Nicotinsäure dosages for combining means, set based on the pharmacokinetics and pharmacodynamics of the individually prescribed or certain means, said adjusting a initial dosage an modifying the combination dosage according to the results of the sensitivity warum Psoriasis auf dem Kopf and includes an in vivo efficacy.
Die Blutprobe kann von einer Click at this page oder einem Lager erhalten werden.
The blood sample may be obtained from a blood bank or stock. Amino zu Carboxyl Psoriasis und Nicotinsäure von links nach rechts gelesen.
Amino to carboxyl is read from left Psoriasis und Nicotinsäure right. The total amino acid content in the expressed protein consists of residues. This invention describes specific anti-chlamydial agents, which are used in combination, to affect more than one of the different phases of the life cycle of chlamydia species. Go here concepts of sensitivity testing for chlamydia and an anti-chlamydial therapy for their associated infections speak only of a phase replicating phase.
For purposes of Psoriasis und Nicotinsäure invention comprises "cryptic phase" means any non-replicating intracellular form of which occur a number of distinct stages, including, but not limited to intracellular EBs, EBs, which transform into RBs and vice versa, miniature RBs, non-replicating RBs and the like.
Are described in detail below diagnostic and therapeutic procedures for the handling of chlamydial infections. Chlamydia infections can therefore be handled by a Psoriasis und Nicotinsäure approach, referred to herein as a "combination therapy", which Psoriasis und Nicotinsäure defined for purposes of this application as the administration of several means, which together at least two, preferably several of the numerous stages of life cycle of Chlamydia, are directed, each agent separately, it can be taken over the course of therapy Psoriasis und Nicotinsäure or sequentially.
When used alone, these agents click the following article chlamydia do not eliminate or not handle. The diagnostic method described below Akne und Psoriasis combination therapies are applicable generally to infection, which is caused by any Chlamydia species, including, but not limited to pecorum, C.
Infektionen, bei denen das verursachende Agens C. Infections in which the causative agent is C. The anti-chlamydial agents Psoriasis und Nicotinsäure have been identified by the sensitivity test method described herein to be effective against chlamydia, can be used individually in order in a single stage to influence Chlamydia their life cycle or as part of a combination therapy, a chlamydial infection to handle.
For example, compounds that have been identified as anti-cryptic phase drugs anti-EB phase drugs, anti-DNA-dependent RNA polymerase drugs and nicotinic acid Kogener-drugs may be used alone or in combination, one or more to eliminate the distinct phases of Psoriasis und Nicotinsäure life cycle of Chlamydia to reduce or prevent. So far no anti-chlamydial activity has been demonstrated for Psoriasis und Nicotinsäure of these compounds.
Diagnose einer Chlamydien-Infektion Diagnosis of chlamydial infection. The invention provides a basis for methods for diagnosing the presence of Chlamydia in a biological material prepared, as well as the use of this process for judging the serological Psoriasis und Nicotinsäure of an individual undergoing an anti-chlamydial combination therapy. For the purposes of this application includes "biological material", but is not limited to body secretions, body fluids or fluids and tissue samples.
Examples of bodily secretions include cervical secretions, and pharyngeal http://gl-dd.de/schlammbehandlung-fuer-psoriasis.php trachealbronchiale secretions.
Suitable body fluids include blood, sweat, tears, cerebrospinal fluid of the system, serum, sputum, ear wax, urine, synovial fluid and saliva. Tiere, Zellen und Gewebeproben, wie beispielsweise von zahlreichen Biopsien werden durch diesen Begriff umfasst. Animals, cells and tissue samples, such as by numerous biopsies are encompassed by this term. There are peptide-based tests for the detection of one or more immunoglobulins such as IgG, IgM, IgA, and IgE disclosed which are directed against antigenic determinants within the recombinant full-length MOMPs different chlamydial species.
IgA determinants are useful in the analysis of humoral article source against Chlamydia in secretions from mucosal surfaces Psoriasis und Nicotinsäure, lung, gastro-intestinal tract, Gerontourin tract, etc.
Similarly, IgE determinants in the analysis of allergic manifestations of disease are useful. Tabelle 1 unten stellt die Zugangsnummern der Gen-Bank von verschiedenen MOMPs von Chlamydien-Spezies bereit. Table 1 below provides the accession numbers of gene bank of different MOMPs of chlamydial species. Tabelle 1 Table 1 Spezies Species Stamm Strain ID ID Gen-Bank Zugangsnr.
Suitable Psoriasis und Nicotinsäure include immunological methods such as enzyme linked immunosorbent assays ELISAincluding luminescence tests eg, fluorescence and Psoriasis und Nicotinsäureradioimmunoassay and immunohistochemistry. In general, a sample and an antibody are combined under conditions suitable for the formation of an antibody-protein complex, wherein the formation Psoriasis und Nicotinsäure an Psoriasis und Nicotinsäure complex directly or indirectly is judged.
Luminescer direkt markiert werden. In all diagnostic methods described herein, the antibody with an enzyme, fluorophore, radioisotope or phosphor or Luminescer can be directly labeled.
Alternatively, antibodies with a specific radical scavengers, such as biotin may be covalently bound. Eine nachfolgende Detektion erfolgt durch eine Bindung von Avidin oder Streptavidin, das mit einem Anzeige-Enzym, Fluorophor, Radioisotop oder Luminescer markiert ist. A subsequent detection carried out by the binding of avidin or streptavidin that is labeled with an indicator enzyme, fluorophore, radioisotope or Luminescer.
In this regard, the step of detection would be carried out by an enzyme reaction, fluorescence, radioactivity or luminescence emission. The antibody Psoriasis und Nicotinsäure be a polyclonal or monoclonal antibody, such as a monoclonal anti-human IgG or a monoclonal anti-human IgM. Examples of useful antibodies include monoclonal mouse anti-human IgG, which do not with other immunoglobulins Pharmingen, clone Psoriasis und Nicotinsäure, catalog no. It can be http://gl-dd.de/psoriasis-nachauflaufbehandlung.php immunoassays, Psoriasis und Nicotinsäure using monoclonal or polyclonal antibodies which do not cross-react with antigenic determinants on MOMP a Chlamydia species of interest, which are specific for Chlamydia or species specificity, but not necessarily a strain specificity within a species provide.
Es wurden auf Psoriasis und Nicotinsäure basierende Psoriasis und Nicotinsäure Tests entwickelt, um die Anwesenheit von Immunglobulinen gegen Chlamydien-Spezies zu quantifizieren. There have been developed based on recombination immunological assays to quantitate the presence of immunoglobulins against chlamydial species. Recombinant full-length chlamydial MOMP may be performed using a suitable expression system, for example, in E. The expressed protein thus serves as the antigen, that is suitable for immunological methods set forth above.
Protein based immunological methods can be developed that are species- and strain-specific for different chlamydia. Any known method Psoriasis und Nicotinsäure amplification of a nucleic acid e. Preferred amplification methods are the polymerase chain reaction PCR method, which comprise a solvent and a PCR in situ PCR to detect the presence or absence of Chlamydia genes unique.
Species-specific Psoriasis und Nicotinsäure for the detection of Chlamydia can be Psoriasis und Nicotinsäure based on the selected primers. Beispiele geeigneter Primer einer PCR-Amplifikation sind nachfolgend in Tabelle 2 dargestellt. Examples of suitable primer of a PCR amplification are shown below in Table 2. Beispiele bevorzugter Primer sind in Tabelle 3 dargestellt. Examples of preferred primers are shown in Table 3.
An amplification of the entire MOMP gene is useful for mutation analysis and the production of recombinant MOMP. For specific amplification of the largest part of the MOMP genome shorter primers may be used with a modification of the amplification protocol. Similarly, smaller regions of MOMP by a wide variety of primer pairs for diagnostic purposes can be amplified, although the benefits of a strain identification is reduced, and amplification may be blocked if one or both pairs of primers hybridize to a region that has been mutated.
Extensive practice with the full-length MOMP PCR amplification shows that mutational events in the hybridization sites CHLMOMPDB2 and CHLMOMPCB2 rare or Psoriasis und Nicotinsäure. The method for Polyurie, Pruritus acid amplification described above can be used to judge the course of an anti-Chlamydia treatment. The absence of detectable resistant Chlamydia DNA MOMP encoded as a function of anti-chlamydial therapy, shows the clinical management of Chlamydia infection.
A serological improvement can be based on current serological criteria for eradication of chronic Chlamydia, which are subsequently reported in Table 4.
Tabelle 4 Serologische Kriterien zur Ausrottung einer chronischen Infektion mit Chlamydia pneumoniae Table Psoriasis und Nicotinsäure. Preferred PCR method set forth below in the Examples section.
Generally, a solution-PCR is performed on a biological material, wherein first the material is pre-incubated in a suitable reducing agent Psoriasis und Nicotinsäure of reducing disulfide bonds that maintain the integrity of the MOMP and other surface proteins of the elementary bodies of Chlamydia, whereby the outer protein coat of the EBs impaired and a Proteasedurchdringung is enabled.
Suitable concentrations of reducing agents the Psoriasis und Nicotinsäure reducing agent can be determined simply Psoriasis und Nicotinsäure a guideline by those skilled in the art without undue experimentation using a 10 uM concentration of dithiothreitol.
Will be omitted, that a reducing agent is included to the initial step, it can be prevented that in the subsequent step DNA isolated from EBs. Data shown in Example 1, show the effects of different reducing agents on the sensitivity of EBs compared to a proteinase K digestion. The in vitro data show that dithiothreitol is extremely effective when opening EBs for a protease digestion.
Once the outer shell of the EBs was superseded or terminated, the preincubated material is a protein digestion using Psoriasis und Nicotinsäure protease eg, proteinase Kor a Psoriasis und Nicotinsäure equivalent enzyme subjected. The DNA is extracted and a method Psoriasis und Nicotinsäure nucleic acid amplification such as a PCR Psoriasis und Nicotinsäure. Die amplifizierte DNA wird dann getrennt und durch standardisierte Elektrophorese-Verfahren identifiziert.
The amplified DNA is then isolated and identified by standard electrophoresis continue reading. The DNA bands are identified using an ethidium bromide staining and detection with UV light. It can be designed primers for this purpose, which can be up to mer of about Psoriasis und Nicotinsäure about. Suitable reporter molecules are well known and can be used here. Es werden jedoch mit Biotin markierte Primer bevorzugt.
However, it is preferred biotin-labeled primers. Alternatively, other specific DNA sequences may be employed, although the sequence described above is the preferred Psoriasis und Nicotinsäure, since the product produced large 1. Alternatively to the labeling habe Jucken der Bauchspeicheldrüse slows the product-specific hybridization probes can be used at Psoriasis und Nicotinsäure regions of the amplified DNA to identify an amplified product.
The present invention provides a base for a unique approach to generating and maintaining animals and cell lines that are free from infection with chlamydia. Also, method for producing of nutrients and culture media described herein Psoriasis und Nicotinsäure are suitable for use with animals and cell lines that Psoriasis und Nicotinsäure been released from an infection with chlamydia. Versuche Isolate von C. Experiments isolates of C. These included not only in-house stock cultures of HeLa, HL, H, HuEVEC and McCoy cells, but also stock cultures from the American Type Culture Collection ATCCthe Research Foundation of the University of Washington for HL cells, as well as real estate professional Bartell of H and McCoy cells for clinical culture of Chlamydia.
Die Anwesenheit einer kryptischen Form von C. The presence of a cryptic form of C. Nevertheless led with fluorescein labeled mAb against LPS in McCoy cells to no display of Psoriasis und Nicotinsäure that is reactive with all chlamydiaePsoriasis und Nicotinsäure fluorescein labeled mAb against C.
These results solution-PCR, in situ PCR, mAb reactivity with an cryptic non-replicating infection of cells by C. Furthermore, almost all have tested to date untreated rabbits and mice on PCR signals for the C. This creates a currently unrecognized problem of utmost importance for those clinical laboratories that Psoriasis und Nicotinsäure cultural services to C.
Clinical and research laboratories currently have no chance to determine whether an organism is actually free of chlamydia. For illustrative purposes here, wild Stress verursacht Schuppenflechte Easy method is described for clarifying cells and animals of C.
A clarifying them conditional, that brought the infected organisms in contact with agents used singly or in combination to eliminate more than one of the different phases of the life cycle of Chlamydia species or so disturbing to intervene. Media change performed every 3 days or twice a week. The cells can Psoriasis und Nicotinsäure 1 to 7 days before they are used for culture purposes or be removed from the protective solution between.
These methods now allow various chlamydial free CF organisms, including resistant cell lines, called to generate HeLa CF, HL-CF, HCF, HuEVEC-CF, McCoy CF, African green monkeys and other Psoriasis und Nicotinsäure lines Psoriasis und Nicotinsäure can support growth of chlamydia.
Various CF strains of mice, rabbits, and other animal models for research are made. Since Chlamydia are highly infectious, have organisms that have been cleared from the extracellular replicating and cryptic infections can be protected from exposure to viable EBs, if the organisms are to remain unloaded. The inventors have found that many of the nutrients and other materials that are used to maintain stable cell lines were contaminated with viable Chlamydia EBs.
For example, each batch of fetal calf serum von Psoriasis tested positive for the MOMP gene of Chlamydia. Since an extensive digestion is required for Psoriasis und Nicotinsäure of DNA, it was concluded that it is bound in EBs. Consequently, it is necessary to inactivate the EBs contained in those materials which are such as culture media and nutrients for maintaining the organism used in a chlamydia-free state.
Gemeinsam werden jene Materialien hier als "Haltungsmaterialien" bezeichnet. Together, those materials are referred to herein as "attitude materials". In one embodiment, nutrients and a culture media are subjected to gamma irradiation to inactivate located therein chlamydia. Preferably, the material should be irradiated for Psoriasis und Nicotinsäure time sufficient to at least 10, rad subjecting the material to gamma radiation.
For the material, it is important that it is stored in containers that do not absorb radiation hochemergetische. The preferred container is plastic. In another embodiment, the conservation materials having a disulfide reducing agent eg, 10 uM dithiothreitol for about 30 minutes are treated, after which the Psoriasis und Nicotinsäure materials by a standardized submicron eg, about 0.
The reducing agent causes Psoriasis und Nicotinsäure EBs expand to a size at which a 0. In yet another embodiment, the conservation materials having a disulfide reducing agents are treated, preferably dithiothreitol eg, about M concen trationbefore the materials are passed through a filtration system to remove therefrom chlamydia. To ensure that research tools, such as cell lines and animals beleiben chlamydia-free, Psoriasis und Nicotinsäure test was developed for judging Psoriasis und Nicotinsäure an organism is chlamydia free.
The method comprises obtaining a sample of cells or a tissue culture, optionally culturing the cells in the presence or absence of cycloheximide, and determining the presence or absence of nucleic acid of Chlamydia Psoriasis und Nicotinsäure a suitable amplification methods such as PCR. The absence of a Amplifikationssignals for nucleic acid indicates that the state of the organism is Chlamydia-free. Empfindlichkeits-Test zur Beurteilung aktiver Mittel gegen verschiedene Formen von Chlamydien Sensitivity test to assess active agents against various forms of Chlamydia.
This Kardashian es wahr, Kim Psoriasis dass ist provides a basis for new approaches to sensitivity testing of Chlamydia species available, which is required by the complex life cycle of Chlamydia pathogen, as well Psoriasis und Nicotinsäure through its diverse, extensive and heretofore unappreciated or understood ability chronic cryptic and prolonged systemic infections cause that are refractory to treatment with short term work conventional single-agents.
The invention is based on the finding that current susceptibility test methods for chlamydia, the ability of anti-microbial Psoriasis und Nicotinsäure not accurately predict that they eradicate chronic chlamydial infections successfully or complete.
Based on the fact that the current susceptibility test methods only detect the replication of Chlamydia and ignore the well-known "cryptic phase," not actively replicate in the intracellular Chlamydia. Es wurde ebenfalls festgestellt, dass die so genannte "kryptische Phase" von Chlamydien mehrere und verschiedene Unterphasen umfasst. It was also found that the so-called "cryptic" phase of chlamydia has several and various phases. The following are some of the phases of the life Psoriasis und Nicotinsäure of Chlamydia that do not replicate the intracellular Chlamydia: Um die kumulative und langzeitige Wirkung einer anti-mikrobiellen Therapie auf diese zahlreichen Lebensphasen zu beurteilen, wurden einzigartige in vitro und in vivo Empfindlichkeits-Test-Verfahren entwickelt und werden hier beschrieben.
To assess the cumulative and long-term action of a anti-microbial treatment on these various Psoriasis und Nicotinsäure of life, have been developed unique in vitro and in vivo sensitivity test method, and are described herein. Der wie Psoriasis und Nicotinsäure verwendete Ausdruck "Empfindlichkeit" ist vorgesehen eine physiologische Antwort eines Organismus auf einen Umgebungs- oder chemischen Stimulus zu bedeuten. The term "sensitivity" as used herein, there is provided a physiological response of an organism to mean an environmental or chemical stimulus.
The desired physiological Psoriasis und Nicotinsäure to stimuli is that it affects the viability of replicating or unstoppable in the host cell pathogen adversely or unfavorable and ideally ie death of the pathogen leads to the reduction or complete extinction.
In Vitro Methodologie A. Here, methods for assessing the sensitivity of the various phases and stages of the life cycle of Chlamydia, in particular the cryptic phase, to a given agent s described, since previously the prior art methods failed the need for drugs to be assessed, the infected Psoriasis und Nicotinsäure can Psoriasis und Nicotinsäure cryptic chlamydia. A drug-By-Musterungs method which comprises solved this task, Psoriasis und Nicotinsäure tissue culture cells, which are held in the absence of cycloheximide to support a cryptic infection.
A cryptic infection is uncommon in cells that are used in standard cell culture sensitivity tests because chlamydia in paralyzed with cycloheximide cells do not need to compete with the host cell to metabolites and are supported to replicate consequently. Das in vitro Verfahren verwendet standardisierte Gewebekulturzellen jedoch ohne Psoriasis und Nicotinsäure Zugabe von Cycloheximid.
However, the used method standardized in vitro tissue culture cells without the addition of cycloheximide.
In addition the Chlamydia is allowed for Psoriasis und Nicotinsäure days prior to the addition of one or more test agents to replicate.
A "test agent" may be any compound or combination of compounds, which is judged as an anti-chlamydial agent for its Psoriasis und Nicotinsäure to reduce the Psoriasis und Nicotinsäure of Chlamydia in living cells significantly. A test agent may include, for example antibiotics, antimicrobial agents, click the following article agents, anti-malarials, disulfide reducing agents and anti-mycobacterial agents include, but is not limited thereto.
Finally, after the end point of the extended incubation period is the complete read more of Chlamydia DNA, as determined by an amplification method of a nucleic acid, for example the polymerase chain reaction PCR methodology is determined.
Standardized amplification technique PCR for nucleic acid can be used to determine the presence or absence of signal for chlamydial DNA encoding MOMP or other unique chlamydial gene to determine whether the test agent or combination of agents in the reduction of chlamydial infection is effective.
This can Psoriasis und Nicotinsäure achieved by carrying out the sensitivity test by the cells are disposed under stringent environmental conditions which are known to induce Chlamydia to enter a Psoriasis und Nicotinsäure phase. Means which, as determined by the susceptibility Psoriasis und Nicotinsäure protocols described herein, are effective against chlamydia, can be used Psoriasis und Nicotinsäure part of a Psoriasis und Nicotinsäure for the handling of infections with Chlamydia.
Appropriate treatment protocols Psoriasis und Nicotinsäure described in Psoriasis und Nicotinsäure below, with a special focus is made on a goal oriented agent or agents Psoriasis und Nicotinsäure against specific stages of the life cycle of Chlamydia.
The methods Psoriasis und Nicotinsäure here are unique in that they assess the activity of antimicrobial agents in the absence of cycloheximide, which is a more clinically relevant intracellular milieu provides.
For example, any normally functioning, energy-dependent membrane pump of host cells can move the antimicrobial agent into or out of the cells inactivated by the Psoriasis und Nicotinsäure of cycloheximide. Die hier beschriebenen Verfahren sind einzigartig, da in ihnen Kulturmedium verwendet wird, das vorher inaktiviert wurde. The methods described here are unique because in them the culture medium is Psoriasis und Nicotinsäure, which was inactivated before.
If a complete eradication of chlamydial DNA was used as an endpoint, then confirmed the susceptibility test, as opposed to merely a temporary stopping the replication that all phases of chlamydia were April Homöopath Psoriasis results. If an amplification procedure for a nucleic acid such as PCR, used to judge the test endpoint, then the nucleic acid test can for example PCR method by the unique application of a reducing agent such as dithiothreitol DTT improved to the sheath disturbing of chlamydial EBs and thus forming DNA may be suspended by the action Psoriasis und Nicotinsäure a protein-digesting connection, such as proteinase K.
In other words, the reducing agent is allowed to break the EB-shell. By the use of a test for DNA, are specifically disclosed in the EBs, the end point of the sensitivity tests evaluated the presence or absence of EBs as well as the presence or absence of both replicating click to see more non-replicating RBs. Ergebnisse, die beispielhaft in diesem Verfahren in vitro erhalten wurden, werden nachfolgend beschrieben.
Results that have been obtained by way of example in this in vitro method will be described below. In one embodiment, a suitable nucleic acid test for identifying agents that are effective against the cryptic form of Chlamydia, in the presence of a test agent ssubjecting comprises cultured cells a reducing agent e. Further, here, a method for identifying cells is described, which include a non-EB cryptic form of chlamydial species by an amplification method e. PCR a nucleic acid comprising, subjecting the cultured cells to a protease digestion, stopping the protease activity, exposing the cells from a suitable heat-stable DNA polymerase, dNTPs and labeled primers e.
Hier wird ein Verfahren zum Identifizieren einer kryptischen Form von Chlamydia beschrieben. Here, a method for identifying a cryptic form of Chlamydia is described. The method comprises treating of cultured cells, of which it is assumed that they are infected Psoriasis und Nicotinsäure chlamydia, with a disulfide reducing agent, subjecting the cultured cells to a protease digestion, exposing the cells to a suitable polymerase, dNTPs and primers for DNA amplification of nucleic acid encoding a Chlamydial protein, exposing the cells to an enzyme reporter molecule, exposing the cells to an appropriate substrate for the reporter enzyme, and determining the presence of a cryptic form of Chlamydia by visualization of the amplified DNA containing a chlamydial protein.
The amplification method can be PCR using the primers may be CHLMOMPDB2 and CHLMOMPCB2 of Chlamydia pneumoniae. Accordingly, the method, the treatment of which are believed to be infected with chlamydia to Psoriasis und Nicotinsäure the chlamydia in the absence of cycloheximide with a disulfide reducing agent, permitting, inclusion of Psoriasis und Nicotinsäure test agent, subjecting includes cultured cells, the cultured cells to a protease digestion, exposing the cells to a suitable polymerase, dNTPs and primers for DNA amplification of a gene encoding a Chlamydial protein, exposing the cells to an enzyme reporter molecule, exposing the Psoriasis und Nicotinsäure to an appropriate substrate for the reporter enzyme and determining the presence of a cryptic form of Chlamydia by visualization of the amplified DNA encoding a Chlamydial protein, such as for example MOMP, coded.
In Psoriasis und Nicotinsäure Methodologie B. Beispielsweise wird ein biologisches Material von dem Menschen oder dem Tier, das einer Kombinations-Therapie unterzogen wird, isoliert. For example, a biological material from the human or the animal which is subjected to a combination therapy, isolated. Das biologische Material wird derart behandelt, dass die Chlamydien davon isoliert werden.
The biological material is treated in such a way that the chlamydia can be isolated from it. Dieses Chlamydien-Isolat gestattet von Chlamydien freie Zellen zu infizieren. This chlamydial isolate of Chlamydia allowed to infect free cells. These infected cells are then exposed to the combination of agents that are used in the individual who is subjected to a combination therapy.
Alternatively, the serum of the individual, the antimicrobial agents to the infected cells contains as a "bactericidal serum test" for intracellular chlamydial infection may be added. The presence of Chlamydia DNA is then detected. In the in vivo method, the mouse model is used, although other animals such as rats or rabbits can be used. The inventors confirmed Psoriasis und Nicotinsäure work of Young and colleagues J.
The inventors have found that these systemic spread also leads to the presence of EBs in the blood of mice. A systemic infection can also be confirmed and monitored by the presence of elevated antibody titers of IgM and IgG. Nachdem die systemische Infektion einer Maus hergestellt war, wurden der Maus antimikrobielle Mittel gegeben. After the systemic infection of a mouse was produced, the mouse was given antimicrobials.
This is done most easily by the addition of antibiotics to the drinking water. This method is unique in that it captures the complete eradication of all life cycles of chlamydia in known target organs of the mouse for chlamydial infection. Dieses Psoriasis und Nicotinsäure vivo Empfindlichkeits-Verfahren zeigte, beispielsweise, dass eine antimikrobielle Therapie mit den Dreifach-Mitteln, INH, Metronidazol und Penicillamin C.
Psoriasis und Nicotinsäure showed in vivo sensitivity method, for example, that antimicrobial therapy with triple agents, INH, metronidazole and penicillamine C. In addition, it proved that following to infect a complete eradication of Chlamydia several attempts they healed mice via intranasal inoculation again, were not successful. This suggests that the effective management and complete eradication to the development of protective immunity leads, and those that effective management is thus a way http://gl-dd.de/oxidationsmittel-torf-in-psoriasis-bewertungen.php produce effective immunity.
Running a PCR for Chlamydia DNA in homogenates of other organ systems may be used to determine the Psoriasis und Nicotinsäure of certain combinations of antibiotics in eradicating infection of chlamydia in these organ systems.
A determination of a previous chlamydial infection may be performed by such systems either by biopsy or an increase in antibody radiological imaging.
Alternatively, a previous infection by performing PCR for Psoriasis und Nicotinsäure DNA homogenates at the same organ systems in a similarly inoculated but untreated control population may be statistically determined.
Die organspezifische Empfindlichkeit wird durch Vergleichen der Raten positiver PCR-Tests in Kontroll- und Behandelten-Populationen bestimmt.
The organ-specific sensitivity is determined by comparing the rates of positive PCR tests in control and treated populations. An alternative or complementary method for determining the presence of cryptic chlamydial infection in an animal or cell culture is to suspend the culture compounds that stimulate chlamydia. Such compounds include but are not limited to cycloheximide, corticosteroids such as prednisoneand other compounds that are known to stimulate a reactivation of cryptic intracellular infections and disulfide reducing agent Psoriasis und Nicotinsäure as dithiothreitoland other chemicals which cause EBs are converted into RBs.
Once the cryptic Psoriasis und Nicotinsäure are entered into a more active phase, they may be prepared using standard detection methods, such as visual detection of inclusion, immunochemical detection of chlamydial antigen or reverse transcriptase-PCR can be detected. Anti-Chlamydien Therapie, die gegen die initialen Stadien einer Infektion mit Chlamydien gerichtet ist Psoriasis und Nicotinsäure therapy, which is directed against the initial stages of an infection with Chlamydia.
Es wurde eine Anzahl wirksamer Mittel, die spezifisch gegen die initiale Phase einer Chlamydien-Infektion dh Transition der Chlamydien-EB zu einem RB gerichtet sind, identifiziert. It has a number Psoriasis und Nicotinsäure active agents that specifically against the initial phase of chlamydial infection ie, the Psoriasis und Nicotinsäure to a Chlamydia EB RB are directed identified.
Diese "kryptische" Wachstumsphase, im Unterschied zu der der replizierenden Chlamydien-Mikroorganismen, die die Wirtzellenergie verwenden, involviert Elektronen und Elektron-Transfer-Proteine als auch Nitroreduktasen. This Psoriasis und Nicotinsäure growth phase, in food besser behandeln Psoriasis der Kopfhaut May to that of the replicating Chlamydia microorganisms using the host cell energy involved electrons and Psoriasis und Nicotinsäure transfer proteins and nitroreductases.
On this basis, it was found that the initial phase of infection with Chlamydia against the antimicrobial effects of nitroimidazoles, nitrofurans or other means is sensitive, directed against anaerobic metabolism in bacteria. Nitroimidazoles and nitrofurans are synthetic antimicrobial agents that are grouped together because they both nitro NO 2 - which are ringed-containing structures and similar antimicrobial effects. Diese Wirkungen erfordern einen Abbau des Mittels innerhalb der mikrobiellen Zelle, so dass elektrophile Radikale gebildet werden.
These effects require a reduction of the agent within the microbial cell so that electrophilic radicals are formed. These reactive electrophilic intermediates then damage nucleophilic protein sites, including ribosomes, DNA, and RNA.
Nitroimidazoles and nitrofurans are currently regarded as having no antimicrobial activity against members of the chlamydial species. This lack of antimicrobial activity, however, is based on the fact that conventional methods of sensitivity tests to test only an effect on the replicating form of chlamydial species.
Examples of suitable nitroimidazoles, but are not limited to metronidazole, tinidazole, Bamnidazol, benznidazole, Flunidazol, Psoriasis und Nicotinsäure, misonidazole, Moxnidazol, Ronidazol, Sulnidazol and their metabolites, Psoriasis und Nicotinsäure and derivatives thereof. Metronidazol wird am meisten bevorzugt. Metronidazole is most preferred. Examples of nitrofurans, which can be used include, but are not limited to, nitrofurantoin, nitrofurazone, nifurtimox, Nifuratel, Nifuraden, Nifurdazil, Nifurpirinol, Nifuratron, furazolidone and its metabolites, analogs and derivatives thereof.
Nitrofurantoin wird in der Klasse von Nitrofuranen bevorzugt. Nitrofurantoin is preferred in the class of nitrofurans. Continuously by this application and for purposes of this invention, it is Psoriasis und Nicotinsäure that "metabolites", products of cellular metabolism of a drug in the host eg, human or animal including, but not limited to, the activated forms of Psoriasis und Nicotinsäure include.
The terms "analogues" and "derivatives" are provided isomers to encompass optically active compounds and Psoriasis und Nicotinsäure chemical or physical modifications of a means such that the modification results in an agent that similar or increased, however, compared to the effectiveness of the parent agent no significant decreased activity against chlamydia has from which the analogue or derivative was obtained.
Dieser Vergleich kann unter Verwendung des hier beschriebenen Empfindlichkeits-Tests ermittelt werden. This comparison can be determined using the herein described sensitivity tests. To be treated cells may be already infected cryptic or they may be subjected stringent metabolic or environmental conditions that determine the replicative phase or induce cryptic phase Psoriasis und Nicotinsäure enter. A unique class of anti-chlamydial agents which are effective against http://gl-dd.de/die-stellen-die-schuesse-bei-psoriasis.php cryptic replicating and stationary phases of chlamydia and possibly against several other stages of the cryptic phasewere, by using the sensitivity tests described herein have been identified.
This new class includes isoniazid INHisonicotinic acid also known as niacinnicotinic acid, pyrazinamide, ethionamide and Aconiazid, INH is most Psoriasis Sommer. Although these are currently being considered go here against mycobacterial infections to be effective, in part because of currently available susceptibility test method, it was found that these agents are particularly effective against chlamydia in combination with other antibiotics.
It is believed that the isonicotinic acid or congener -Artverwandte are directed to the constitutive production of catalase and peroxidase, which is characteristic for a property of microorganisms, such as the mycobacteria that infect mono Zoten and macrophages. Chlamydia can also successfully infect Mono obscenities and macrophages.
INH is used to eradicate Chlamydia of macrophages and mono- obscenities, this subsequently supported these cells to fight the infection in their role.
Diese Mittel scheinen jedoch gegen die kryptische Phase in vitro weniger wirksam zu sein. These agents, however, appear to be less effective against the cryptic phase in vitro. Consequently, ethambutol, and other INH isonicotinic acid congener ideally should be used in combination with agents that are directed at other stages of the life cycle of Chlamydia.
Be infected Mono obscenities and macrophages by chlamydia, then they are weak and can not fight the infection appropriate or Psoriasis und Nicotinsäure. As noted above, it was found that unfavorable conditions, such as, limited nutrients, antimicrobials and host immune response generate stringent responses in Chlamydia.
Antibiotics in Laboratory Medicine, Fourth Edition. Such adverse conditions are known in other microorganisms CW Stratton. Antibiotics in Laboratory Medicine, Fourth Edition Lorian V. These stringent response in Chlamydial changes the morphological Psoriasis und Nicotinsäure of the intracellular microorganism and generates dormant forms, including the intracellular EB that can persist cryptic then to Psoriasis und Nicotinsäure development cycle reactivated.
Conversely, the lysed host cell, which allows the EBs to reach the extracellular milieu. Consequently, it is necessary to use a combination of agents that are directed against various life stages of chlamydia and in particular for a successful handling of infection against the elementary bodies. While the unique life cycle of Chlamydia is known to be metabolically inactive spore-like EBs are released into the extracellular milieu. Although these liberated EBs are infectious, Psoriasis und Nicotinsäure can not directly infect neighboring sensitive host cells, unless appropriate conditions for EBs infectivity are available.
The result of such delay in infection consists metabolically inactive in the extracellular accumulation, still infectious EBs. This expression is the concept of an HIV strain similar and is used here as the number of algopiks Shampoo Psoriasis EBs defined, which are located in the extracellular milieu. Direct Microscopic Visualisation method, tissue culture and testing of the polymerase chain reaction showed that infectious EBs are often read more in the blood of apparently healthy people and animals.
This phenomenon has obvious great clinical importance in chlamydial infections, because they metabolically inactive EBs the action of a current anti-chlamydial escape therapy, which are only directed against the intracellular replicating forms of chlamydia.
The presence of infectious extracellular EBs after completion of a short-term, anti-replicating phase therapy against infections with Chlamydia showed that it leads to a relapse of an intracellular infection. Consequently, the duration and nature of an anti-chlamydial therapy, which is required for handling of chlamydial infections is partly dictated by the extracellular burden of EBs. For purposes of this invention, Psoriasis und Nicotinsäure short-term therapy, a long-term therapy may be about two to three weeks, whereas several months.
Wie in vorherigen Abschnitten beschrieben Psoriasis und Nicotinsäure ebenfalls angenommen, dass die Persistenz von Chlamydien-Infektionen teilweise auf der Anwesenheit von kryptischen Formen von Chlamydien in click the following article Zellen beruhen kann. As described in previous sections, it is also assumed that the persistence of chlamydial infections can be due in part to the presence of cryptic forms of chlamydia in cells.
This cryptic intracellular Chlamydia form can apparently caused by certain host factors, such as cortisone Yang et al. An anti-chlamydial therapy for chronic chlamydial infections here be continued Psoriasis und Nicotinsäure any intracellular EBs or other intracellular cryptic Psoriasis und Nicotinsäure were activated and extracellular EBs were infected host cells.
It biochemical agents were identified, read article chlamydial EBs in the respective hosts by reducing disulfide bonds Psoriasis und Nicotinsäure maintain the integrity of the outer membrane proteins of EB inactivate.
With the destruction of chlamydial outer membrane proteins of EBs initiated the transition from the EB-form to the RB form. This from happening in the acellular milieu, where there are no available source of energy is the emerging RB basis or is the victim of the immune system. Consequently disulfide reducing agents which may interfere with this process, suitable as compounds for the elimination of EBs.
Eine derartige Klasse von reduzierenden Disulfid-Mitteln sind Thio-Disulfid-Austauschmittel. One such class of reducing agents are disulfide-thio-disulfide interchange means. A particularly Psoriasis und Nicotinsäure extracellular anti-chlamydial within this class is DMSA, which is a chelating agent having four ionizable hydrogens and two highly charged carboxyl groups, which prevent Psoriasis und Nicotinsäure relative or a passage passing through the human cell membranes.
DMSA therefore remains in the extracellular fluid, where it can easily meet extracellular EBs. Penicillamin kann ebenfalls als reduzierendes Disulfid-Mittel verwendet werden, um Chlamydien-EBs zu beseitigen. Penicillamine can also be used as a reducing agent disulfide to eliminate chlamydial EBs.
However, the use of penicillamine can cause undesirable side Psoriasis und Nicotinsäure. Currently approved agents are active against chlamydial replication Since transform chlamydial RBs in EBs, they begin an active transcription of chlamydial DNA and translation of the resulting mRNA to use.
As such, these forms of Chlamydia over currently used anti-chlamydial agents are sensitive. The anti-chlamydial activity of these agents may be improved by their use in combination with other agents, which are directed against different stages of life-cycle of Chlamydia, as embodied. Beispiele von diesen Mitteln, die Mitglieder dieser Klassen sind, als auch solchen, die bevorzugt sind, Psoriasis und Nicotinsäure nachfolgend in Tabelle 5 dargestellt.
Examples of these agents, the members article source these classes, as well as those which are preferred are shown below in Table 5.
Rifampin Rifabutin Rifabutin rifampin Rifampin Rifampin. It is assumed that all members of the Chlamydia species, including C. Using the new sensitivity tests, the inventors have found, however, that complete eradication of Chlamydia can not be Psoriasis und Nicotinsäure by any one of Psoriasis und Nicotinsäure agents alone because none against all stages of the life cycle of Chlamydia is effective and seemingly stringent response in chlamydial induced, thereby transforming the replicating phase is due to the cryptic phase.
This leads to persistent infection in vivo or in vitro, can be represented by the PCR method that judges the presence or absence of Chlamydia DNA. Nevertheless, should one or more agents currently used by these, or a new agent that is directed against replicating phase Chlamydia, be included as one of the chlamydial agent in a combination therapy to slow or stop the transition of EB to RB as well as to inhibit the chlamydial replication. In an attempt Psoriasis und Nicotinsäure manage or eradicate systemic Psoriasis und Nicotinsäure, it is crucial to in, was scheint, Psoriasis at several stages in the life cycle of Chlamydia, while otherwise viable Chlamydia in phases non-directional will remain after the treatment, resulting in a continuous, chronic infection.
Diese fundamentale Einsicht ist der Kern dieser Erfindung. This fundamental insight is the core of this invention. A preferred method for selecting an appropriate combination of agents that meet the requirements of this strategy includes, as follows several steps: Identifizieren der Phasen des Lebenszyklus der Chlamydien. Identify the phases of the Psoriasis und Nicotinsäure cycle of Chlamydia. For example, the following phases are currently known: Intracellular EBs may represent a type of "cryptic" phase.
EB transition phase in reticulate bodies "RB". This ensures that constitute what is commonly called the "cryptic" phase considered. RB zu EB Transitionsphase ebenfalls als "Kondensation" bezeichnet. Beurteilen der relativen Wichtigkeit eines Abzielens auf jede besondere Phase beim Ausrotten von Reservoirs vom Chlamydien aus dem Wirtsorganismus.
Assess the relative importance of Abzielens more info each particular phase in eradicating reservoir of chlamydia from the host organism.
For example, those listed in step 1 Psoriasis und Nicotinsäure cycle stages are given based on the following assumptions priority: Most intracellular RBs in a chronic infection are not replicating.
The day replication cycle, which is seen in patients treated with cycloheximide eukaryotic cells, is an artifact of an atypical cell culture environment, which was mainly developed Psoriasis und Säfte increase chlamydia.
Die Transitionsphasen stellen Psoriasis und Nicotinsäure einen kleinen Anteil bei chronischen Infektionen von Chlamydien dar. The transition phases represent only a small portion is in chronic infections of chlamydia. Identifizieren von "Zielen bzw. Identify "goals or targets" for each phase of this web page selected life cycle phases.
An object is a characteristic of Chlamydia, which is vulnerable during a specific life cycle stage. For example, set the disulfide bonds in the MOMP a goal during the EB phase.
Identifizieren von Psoriasis und Nicotinsäure mit einem bekannten oder theoretischen Wirkungsmechanismus en gegen solche Ziele. Press the judgment by use of the following approaches: Click at this page the case of anti-EB-agents, treating EBs with the agent then try cells with the treated EBs to infect.
If the Psoriasis und Nicotinsäure are not infected, then the agent is EB-killing. In the Psoriasis und Nicotinsäure of other agents, the sensitivity tests are used, which flaxseeds Psoriasis disclosed elsewhere herein, to determine whether the agent is Chlamydia killing either alone or in combination with other agents.
Preferably, a combination of as many phases should aim of the life cycle read article possible, to try all of the relatively important point rating or score thereon abgezielten phases to maximize, while the number of drugs concerned is minimized.
Testen der Kombination unter Verwendung der an anderer Stelle beschriebenen Empfindlichkeits-Testverfahren. Testing of the combination using the described elsewhere susceptibility test method.
Setting of initial doses, based on clinical standards, taking into account the pharmacokinetics and pharmacodynamics of the drug individually described, wherein, if necessary, modifications on results of the sensitivity tests and based in vivo efficacy.
Table 6 shows an example prepared as the above Psoriasis und Nicotinsäure may be used. The preferred embodiment comprises means for: The above methodology for selecting combination therapies may be in one or a combination of the above-described steps automatically for example, by a computer system.
Psoriasis und Nicotinsäure methodology is applicable even for a better understanding of the life cycle of chlamydia, which leads to a re-prioritization, or this web page a sub-division of the life cycle phases, wherein, new theoretical targets are identified within the Chlamydia, or new drugs to be developed, the presently known or new goals in chlamydia attack.
For example, the phases of the life cycle can be based on the host cell type in which the phase is, subclassified. Consequently, the stationary phase RBs could be considered in macrophages as one of the stationary phase RBs in hepatocytes separate phase. Dies gestattet der zu verwendenden Methodologie eine einzelne oder eine mehrfach Gewebe-spezifische Kombination von Mitteln zu entwickeln. This allows to use the methodology to develop a single or a multiple tissue-specific combination of agents.
Krankheiten, die mit einer Chlamydien-Infektion in Verbindung stehen Diseases which are associated with a chlamydial infection. To date, these diseases include multiple sclerosis MSrheumatoid arthritis RAinflammatory bowel disease IBDinterstitial cystitis ICfibromyalgia FM Psoriasis und Nicotinsäure, autonomic nervous dysfunction AND, neurally mediated hypotensionpyoderma gangrenosum PGchronic fatigue CF and chronic fatigue syndrome CFS.
Andere Krankheiten werden untersucht. Other diseases are examined. Eine Korrelation zwischen einer Chlamydien Infektion und diesen Krankheiten wurde erst vor kurzem als Ergebnis, der hier beschriebenen Diagnose-Methodologien und der Kombinationstherapien hergestellt. A correlation between chlamydial infection and Psoriasis und Nicotinsäure diseases has been made recently Psoriasis und Nicotinsäure a result of diagnostic methodologies described herein and combination therapies.
On the basis of this evidence, the publicized evidence of a link between atherosclerosis and Chlamydia Grupta et al, Circulation, Der Verlauf der Behandlung Psoriasis und Nicotinsäure serologisch beurteilt werden, um Psoriasis und Nicotinsäure Anwesenheit oder Abwesenheit von Chlamydien unter Verwendung von beispielsweise der hier bereitgestellten Diagnoseverfahren bestimmt werden, wobei dieser Wert mit serologischen Werten verglichen werden kann, die Psoriasis und Nicotinsäure in der Therapie gemessen wurden.
The physical improvement just click for source conditions and symptoms that are commonly associated with the disease being treated, should also be assessed. Based on this investigation factors, the physician can maintain the anti-chlamydial therapy or change accordingly.
Psoriasis und Nicotinsäure example, replacing an agent due to adverse side effects, Psoriasis und Nicotinsäure are caused by the means of inefficacy or other reasons, the doctor.
When increasing the antibody titer during treatment, to varying connections are replaced in order to achieve the low antibody titers, which show the specific sensitivity of Chlamydia against the new regime. An exchange or a substitution of an agent against another agent that is effective against the same life stage of Chlamydia is desired.
The therapies described here can therefore be Psoriasis und Nicotinsäure for the treatment of acute and chronic immune and autoimmune diseases when patients enjoy a stress was found with chlamydia by the diagnostic methods described herein comprise diseases, but not limited to, chronic hepatitis, Psoriasis und Nicotinsäure lupus erythematosus, arthritis, Thyroidose, scleroderma, diabetes mellitus, Graves disease.
The therapies this web page herein can also be used to treat any disorders in which a chlamydial Psoriasis und Nicotinsäure is a factor or co-factor.
Among the various inflammatory diseases, there are certain properties of the inflammation Psoriasis und Nicotinsäure, which are generally recognized characteristic.
These include the fenestration of the microvasculature, leakage of blood elements into the interstitial space and migration of leukocytes into the Psoriasis und Nicotinsäure tissue. At the Psoriasis und Nicotinsäure level, this is often accompanied by the familiar clinical signs such as erythema, edema, painful tensions hypersensitivity and pain.
The invention can also be used for the treatment of inflammatory diseases, such as coronary artery disease, hypertension, stroke, asthma, chronic hepatitis, multiple sclerosis, peripheral neuropathy, chronic or recurrent sore throat, laryngitis, tracheobronchitis, chronic vascular headaches including migraines, cluster Tension headache and headachesand pneumonia when it was shown that the disease is based on a pathologically chlamydial infection.
Ocular neovascularization, psoriasis, duodenal ulcers, angiogenesis of the female reproductive tract can also be treated if indicated by the diagnostic method described here is that they are associated with chlamydial infection.
An immunocompromised individual is generally defined as a person who exhibits an attenuated or reduced ability to build a normal cellular or humoral defense against infectious agents such as viruses, bacteria, fungi and protozoa.
Persons who are considered with a weakened immune system, include malnourished patients, patients undergoing surgery and received a bone marrow transplant patients receiving chemotherapy or radiotherapy, neutropenic patients, HIV-infected patients, trauma patients, burn injured patients, patients with chronic or resistant infections, such as those resulting from myelodysplastic syndrome, and the elderly, all of whom have a weakened immune system. A protein malnourished individual is generally defined with as a person who has a serum albumin level of less than about 3.
The course of the therapy, the results of serological and clinical improvements by a participating anti-chlamydial therapy in patients als Ihr in Psoriasis FARBSTOFF have the cited diseases, have been observed and reported in Example 5. The data provide evidence prepared to justify the statement that the treatment of Psoriasis und Nicotinsäure infection Psoriasis und Nicotinsäure serological and physical this web page of a disease state of a patient leads, passing through the combination therapy.
Diese Beobachtungen waren einheitlich unter einer Vielzahl von verschiedenen Erkrankungen, die unter eine generalisierte Krankheitsklasse fallen. These observations were consistent with a variety of different diseases that fall under a generalized disease class. Is a previously known basis for this difference in the amino acid sequence of the MOMP. Note that the size and sequence are relatively click the following article except for the four variable regions for the serovar serotypebased on the classification, are responsible.
Weiter wurde gefunden, dass C. Further, it Psoriasis und Nicotinsäure found that C. In addition, macrophages are known targets of C. Infected sites can over a longer period of time without symptoms are induced exist that are detected by the click the following article or an examining physician. The sequence variability of MOMPs and other chlamydial antigens may provide a basis for an organ specificity, while other chlamydial proteins, such as the K or kDa and 70 K heat-shock proteins or LPS, can affect the immune response.
Therefore, the teachings of this invention are relevant for animals. Throughout this application, and for purposes of this invention, is intended to include click the following article includes both humans, and animals.
Almost all rabbits and mice that were previously tested exhibit PCR signals for C. They Psoriasis und Nicotinsäure be used as suitable animal models for the treatment using specific combinations of antibiotics to improve the therapy. Rank et al, Amen J. Fong et al, J. Clin Microbiol 35 Verbunden mit diesen Entwicklungen sind die in letzter Zeit entwickelten Kaninchenmodelle der koronaren Arterienerkrankung, wobei Kaninchen nach einer Exposition mit C. Associated with these developments, the recently developed rabbit models of coronary artery disease, with rabbit arterial after exposure to C.
Vor kurzem wurde durch eine Studie an dem St. Recently, it was found by Psoriasis und Nicotinsäure study of the St. Zusammengenommen liefern diese drei Hinweise die Bakterien, die in erkranktem Gewebe aufgefunden wurden, Beimpfung bzw. Taken together, these three statements the bacteria that were found in diseased tissue, inoculation or Psoriasis und Nicotinsäure with these bacteria leads to disease and treatment against the bacteria alleviates disease arguments for a causal relationship.
The initial supply of an anti-inflammatory steroid can be introduced to minimize side effects of anti-chlamydial therapy in those patients in whom the clinical judgment suggests the possibility Psoriasis und Nicotinsäure a severe inflammatory complication is. Suitable anti-inflammatory agents steroidal and non-steroidal agents include, but are not limited to, prednisone, cortisone, hydrocortisone, and naproxin. Preferably, the anti-inflammatory agent is a steroid agent, such as prednisone.
The amount and frequency of administration of these compounds will be appended dependent on the health of the patient, his age, clinical status, and other factors just click for source medical experts, obviously. Vitamin C 2 grams offer bid has also been taken on the basis of the report that go here C ascorbic acid at moderate intracellular concentrations stimulates the replication of C.
In addition probenecid may optionally be added as an amplifier of therapy. Chlamydia ist ein Parasit einer normalen Energieproduktion in infizierten eukaryotischen Zellen.
Chlamydia is a parasite of normal energy production in infected eukaryotic cells. As a result of host cells have not enough energy to function normally. The energy shortage may also cause the mitochondria of the host cell to try certain key enzymes that occur in the Psoriasis und Nicotinsäure of energy to synthesize, to increase energy production. Since Chlamydia also prevents Psoriasis. Psoriasis-Arthritis completion of the synthesis, the precursors of these enzymes accumulate, called porphyrins in the cell and often escape into the Psoriasis und Nicotinsäure milieu.
Porphyrins easily form free radicals that damage cells again. Therefore, there is a lot of chlamydial infections accompanying bonded secondary porphyria.
A therapy against this secondary porphyria, which is attached to an anti-chlamydial therapy, at least involves three strategies: A cross-reaction with vitamin B12 can lead to an autoimmune-mediated vitamin B12 deficiency with only minor clinical signs of disease. The first of these concomitant disorders porphyria, which is a Psoriasis und Nicotinsäure result of chlamydial infection of the host cells.
This form of porphyria is a secondary, since it does not result from a genetic malfunction of the enzymes involved in the biosynthesis of heme.
Based on the finding of this second form of Psoriasis und Nicotinsäure is a unique approach to the diagnosis and treatment of compulsory and secondary disorders that are caused by chlamydia infections, have been developed. The add-on therapy described herein can be used in combination with appropriate antimicrobial therapy, which is required for the eradication of the pathogen.
The following discussion deals with the mechanisms adopted, the chlamydia induce the secondary metabolic disorders. The term "by chlamydia-induced porphyria" is herein defined as a mandatory and secondary metabolic disorder, which is the direct result of a this web page infection and the clinical relevance has a phenotypic expression to give an intervening therapy necessary. Chlamydien sind Prokaryoten, die sich in eukaryotischen Zellen entwickeln und Teile des Metabolismus der Wirtszellen nutzen Becker, Y.
Chlamydiae are prokaryotes that develop in eukaryotic cells and parts of the metabolism of the host cells use Becker, Y. The transition of the elementary bodies EBs for reticular bodies RBs for chlamydial species requires the presence of functioning mitochondria in the infected cell, as well as the production click nucleoside triphosphates by the host cell, which are required for the biosynthesis of nucleic acids by the chlamydia Becker, Y.
It is known that chlamydia have parts of glycolysis, pentose phosphate pathway and the citric acid and it looks as if they are in a position to glucosephosphate but not glucose into pyruvate and pentose Ormsbee, and RA Weiss, E.
However, it looks as if chlamydia have no enzymes that are required for the net production of adenosine triphosphate ATP Weiss, E. Therefore, the chlamydial development is dependent on an active mitochondrial and nuclear function of the host cell. For this reason, chlamydia Psoriasis und Nicotinsäure as obligatory intracellular parasites McClairty, G. The dependence of chlamydia from the power of the host cell must reduce the existing energy output of the host cell to net cost of the biosynthetic pathways of the host cell deprived necessarily.
The requirement of an exogenous source of ATP and the presence of a specific ATP transport system in Chlamydiae presented supporting evidence for the energy parasites concept ready Hatch et al, Jour Of Bacteriology, Dieses Psoriasis und Nicotinsäure Transportsystem ist ein Adenosindiphosphat ADP Austausch-Mechanismus Peeling et al.
This ATP transport system is an adenosine diphosphate ADP exchange mechanism Peeling et al, Infect and Immun, In addition, electron microscopy showed that replicating Chlamydia are always found in close proximity to mitochondria. It was therefore suggested that chlamydia in the reverse way to Psoriasis und Nicotinsäure mitochondria by mitochondrial ADP import and export from the host cell Psoriasis und Nicotinsäure, ADP and ATP during import Psoriasis und Nicotinsäure export Becker, Y.
Die Produktion von ATP innerhalb der Mitochondrien wird durch einen Mechanismus angetrieben, der chemiosmotische Kopplung genannt wird Kalckar, HM, Annu. Molecular Basis of Structure and Function, The Benjamin Company, Incorporated, New York; Slater, EC, Europ. The production of ATP within the mitochondria is driven by a mechanism called the chemiosmotic coupling Kalckar, HM, Annu Review of Psoriasis und Nicotinsäure, Molecular Basis of Structure and Function, The Foto Schuppenflechte an Fingern Company, Incorporated, New York; Slater, EC, European Journ of Biochem, Since the high-energy electron pair is transferred to each of the three multi-protein complexes that protons produced by the mitochondria matrix run unhindered through channels in the complexes I, III and IV in the intermembrane space.
Daher Psoriasis und Nicotinsäure der Elektronentransfer von NADH die Elektronentransportkette hinunter, dass die Protonen aus der Mitochondrien-Matrix hinaus in den Intermembranraum gepumpt werden. Therefore, the electron transfer from NADH causes down the electron transport chain that the protons are pumped from the mitochondrial matrix out into the intermembrane space. These protons then pass back through a Psoriasis und Nicotinsäure channel in complex V in the matrix.
Dieser Protonengradient entlang der inneren Membran resultiert in der Protonenantriebskraft, die die ATP-Synthese betreibt. This proton gradient along the inner membrane resulting in proton motive force which operates the ATP synthesis. Die Chlamydien-ATPase konkurriert im Wesentlichen mit der Psoriasis und Nicotinsäure der Mitochondrien um die Protonen der Wirtszelle. The Chlamydia-ATPase competes with substantially the mitochondrial ATPase to the protons of the host cell.
This of course reduces the ATP produced by the mitochondria. A net reduction of ATP in the mitochondria of the host cell leads to a simultaneous reduction of the electron transfer in the mitochondria of the host cell, since the electron transfer and ATP synthesis are directly connected to each other, none of the reactions without the other occurs. The introduction of a large electrochemical proton gradient along the inner mitochondrial membrane normally stops the electron transport and can even cause a reversal in the flow of electrons in some areas of the respiratory chain of the host cell.
The reduction of the electron transfer in the mitochondria of the host cell, in turn, lowers the translocation and reduction in matrix occurring outside the iron III occurring within the mitochondrial matrix of iron II.
This energy loss in turn affects the biosynthesis of heme. The heme-containing tetrapyrrole macrocycle pigments are known as porphyrinogens and play an important role in cellular biochemistry. A number of critical cellular functions, such as electron transport, reduction of oxygen and hydroxylation are mediated by a family of cytochromes Psoriasis und Nicotinsäure on heme, including catalase, peroxidase, and superoxide dismutase.
In addition, based on the oxygen-carrying properties of hemoglobin and myoglobin heme. The biosynthesis of heme Battersby et al, Nature, The metabolic consequence Psoriasis und Nicotinsäure the interruption Psoriasis und Nicotinsäure the heme biosynthesis is porphyria Ellefson, RD, Psoriasis und Nicotinsäure Clinic Proceedings, The heme synthesis is Psoriasis und Nicotinsäure series of non-reversible biochemical reactions, some of which take place in the mitochondria of the Psoriasis und Nicotinsäure, and others in the cytoplasm.
The intra-mitochondrial mainly oxidation-reduction reactions, while reactions taking Psoriasis und Nicotinsäure in the cytosol are condensation and decarboxylation. Porphyrinogens, porphyrins and porphyria affect all the heme synthesis.
The biosynthesis of heme occurs in all human cells and contains a relatively small amount of starting materials, which are condensed to form porphyrinogens, wherein the porphyrins are formed by non-enzymatic oxidation of the Psoriasis und Nicotinsäure. When passing through the porphyrinogens by the heme biosynthetic pathway decreases the number of carboxyl side groups of the corresponding porphyrin, such as the water solubility of the composition.
The porphyrias are consequences of any weakening in the formation of porphyrinogens or in their conversion to heme. Porphyrine werden aus Porphyrinogenen durch nicht-enzymatische Oxidation gebildet. Porphyrins are formed from porphyrinogens by non-enzymatic oxidation. Each of the different genetic porphyria is an enzyme failure in the heme biosynthetic pathway in context. As a consequence of the enzyme defect, there is an increased activity of the initial and the speed Beistimmenden enzyme of this pathway, which leads to an overproduction and increased release of porphyrinogen precursors and porphyrinogens.
The steps of the heme biosynthesis are shown in Table 7. Porphyrinogens enrich themselves due to enzymatic defects in the heme biosynthetic pathway, then they are oxidized to Psoriasis und Nicotinsäure porphyrins. Porphyrins can be Psoriasis und Nicotinsäure to excited molecules of the non-excited after absorption of radiation. Excited porphyrins transfer energy to oxygen molecules and produce reactive oxygen species such as singlet oxygen, superoxide anion, superoxide radical, hydroxyl radical and hydrogen peroxide.
Psoriasis und Nicotinsäure Sauerstoffspezies wurden beim Zerbrechen von Membranlipiden, Cytochrom P und DNA-Strukturen beobachtet. Reactive oxygen species were observed in the breakage of membrane lipids, cytochrome P and DNA structures. If these reactive oxygen species are released into the extracellular space, which has been observed in acute porphyria, it can lead to auto-oxidation of the surrounding tissue.
If Coproporphyrinogen is not able to return to the mitochondrial matrix, it accumulates in the cytosol at first and then into the extracellular milieu. In the mitochondrial matrix, the final steps in the biosynthesis of heme come to a standstill. The depletion of host cells energy through the intracellular infection with Chlamydia species leads to additional energy-related complications. The net result is a higher amount of heme precursors, which are to porphyrins. The Psoriasis-Behandlungen Forum 2015 of porphyrins in the mitochondrial matrix damages the cell, since these molecules are unstable and Psoriasis und Nicotinsäure free radicals.
Click the following article Hoch-Energie-Elektronen, die durch diese freien Radikale erzeugt werden, werden durch Ubiquinon und Cytochrom c "eingefangen", die in der mitochondrialen Matrixmembran vorkommen. The high-energy electrons that Psoriasis und Nicotinsäure generated by these free radicals are "captured" by ubiquinone and cytochrome c, which occur in the mitochondrial matrix membrane.
This decouples naturally effective electron transport from ATP synthesis and "closes" the proton motive force "short": ATP synthesis is then reduced. Less ATP in turn means increased porphyrins and a destructive cycle begins. While infected with chlamydia host cell lysis, as happens in the normal see more cycle of Chlamydia, the intracellular porphyrins Psoriasis und Nicotinsäure released and result in a secondary porphyria.
In addition, when the chlamydial infection involves the liver cells, the use of any pharmacological agents which are metabolized in the liver by cytochrome P, the need for the cytochrome P is increased, which is a heme-based enzyme.
Therefore, the biosynthesis of heme is increased in the liver. It was also observed that any infected with a chlamydial species host cell has an increased amount of intracellular porphyrins which are released when antimicrobial agents kill the microorganism. Although a number of researchers have reported about diprospan für Psoriasis porphyria in patients Psoriasis und Nicotinsäure had no evidence of abnormal enzymes in the heme biosynthetic pathway Laiwah Yeung et al, Lancet, i: This compulsory secondary porphyria is when dealing with chronic systemic chlamydial infections, as seen with intravascular infections caused by Chlamydia pneumoniae, clearly of paramount importance.
The diagnosis of chlamydia-associated secondary porphyria is due to the well-known neuropsychiatric manifestations important Gibson et al, Journal of Pathology and Bacteriology, Von besonderem Interesse ist, dass Infektionen mit Chlamydia pneumoniae mit Lungenkrebs in Verbindung gebracht wurden Cerutti PA, Science, Of particular interest Psoriasis und Nicotinsäure that infections with Chlamydia pneumoniae have been associated with lung Psoriasis und Nicotinsäure PA Cerutti, Science, Die Diagnose von genetischer Porphyrie in Patienten mit systemischen Chlamydien-Infektionen ist wichtig, da diese Patienten ein schwerer porphyrischen bzw.
Porphyr Anfall niederschlagen kann, wenn sie antimikrobielle Mittel zur Behandlung ihrer Infektion erhalten. The diagnosis of genetic porphyria in patients with systemic chlamydial infections is important as these patients may precipitate a severe porphyry or porphyry attack when they receive antimicrobials to treat their infection.
In contrast, the diagnosis of Psoriasis und Nicotinsäure secondary porphyria can be difficult because the porphyria may be minimal and tissue specific. The measurement of hour urine porphyrins is not sensitive enough to detect the secondary porphyria, which was caused by a Chlamydia infection, in each case. Psoriasis und Nicotinsäure view of the previous discussion about the etiology of porphyria aspect of the invention relates to a Psoriasis und Nicotinsäure for differentiation of porphyrias, which are caused by chlamydia, of which, caused Suzuki Psoriasis pustulosa von Handflächen und Fußsohlen two-step a deferred genetic disorder in a subject.
The method comprises treating Chlamydia infection in all stages of their life this web page using the therapies described in detail elsewhere in this disclosure and then judging whether the symptoms of porphyria have been reduced. A reduction in the symptoms of porphyria eg, biochemical, enzymatic or physical manifestations Psoriasis und Nicotinsäure that the porphyria is a secondary porphyria, which was caused by chlamydia.
The diagnosis of genetic porphyria is simply made during an acute porphyric attack since then porphyrinogen precursors and porphyrins in the blood, urine and stool are available Kaupeinen et al, British Journal of Cancer, The diagnosis of secondary porphyria is not as easy to perform as an abnormal amount of porphyrinogen precursors and porphyrins in the blood, urine or stool does not have to be necessarily present.
However, various enzymes present in the heme biosynthetic pathway early, can be easily detected in peripheral red blood cells Percy et al, South African Forensic Medicine Journal, Therefore, increased porphyrin interpret in patients who do http://gl-dd.de/psoriasis-behandlung-tyanshi.php have low levels of these enzymes, a non-genetic porphyria out, such as chlamydia-induced secondary porphyria.
The diagnosis of chlamydia-associated secondary porphyria can be difficult because the porphyria may be minimal and tissue-specific. The detection of Psoriasis und Nicotinsäure urine or stool porphyrins may not be sensitive enough in many cases to detect the secondary porphyria of chlamydial infections.
Here, the diagnosis depends on the fact that the precursors of red blood cells absorb the excess porphyrins and produce heme, when the excess porphyrins reach the circulation. Therefore, increasing the enzymes for the biosynthesis of heme in the differentiated red blood cells and remain elevated for the life of the red blood cell.
This enables the diagnosis of low levels episodic secondary porphyria, as seen in chlamydial infection. Therefore, the increased synthesis of heme-mirrors may be used for the diagnosis of intracellular porphyria. As described above, have some patients with Chlamydia-induced porphyria in no abnormal levels of heme precursors.
Bei diesen Patienten kann es angebracht sein, die Anwesenheit von Chlamydien, sowie von Porphyrinen in dem Individuum zu bestimmen. In these patients, it may be appropriate to determine the Psoriasis und Nicotinsäure of Chlamydia, as well as of porphyrins in the individual.
The presence of both the pathogen, and by the porphyrin determined for example Psoriasis Volksheilmittel Gesicht im für ELISA assay, click is described later is more indicative of a chlamydial secondary porphyria, as for a genetically-based porphyria.
A suitable Psoriasis und Nicotinsäure can therefore determine the http://gl-dd.de/haar-salbe-fuer-psoriasis.php regime that is needed to treat the infection and the symptoms of secondary porphyria.
Psoriasis und Nicotinsäure inventors have investigated the occurrence of antibodies to the different metabolites Psoriasis und Nicotinsäure heme biosynthesis, as well as Psoriasis und Nicotinsäure B12 cobalaminneben machen eine Salbe für Psoriasis now is similar to these molecular metabolites found in patients with an active systemic infection with C.
The antibodies are Psoriasis und Nicotinsäure IgM, which is similar to the antibody responses to the MOMP of C. Beispiel 8 zeigt Titer in symptomatischen Patienten mit systemischen C. Example 8 shows titers in symptomatic patients with systemic C.
The presence of antibodies against vitamin B12 may be functionally important by reducing the amount of biologically available vitamin B Therefore, may cause a previously unrecognized secondary vitamin B12 deficiency chlamydial infection. The administration eg, intramuscular of large amounts of zur Behandlung von Psoriasis Dayvoneks B12 to check this out eg read more cobalamin therapy creates large amounts of vitamin B12, which is available for binding to the native receptor, the antibody having an affinity for vitamin B12, thereby these anti-vitamin B12 antibody saturates and the amount of biologically available circulating vitamin B12 can be increased.
The previously unknown fact that the body produces antibodies against porphyrins, allows the determination of the presence of porphyrins in a patient or animal by determining the presence of anti-porphyrin antibodies. The inventors have developed a method in which IgM and IgG antibodies Psoriasis und Nicotinsäure porphyrins with an ELISA method can be detected.
Es wurde gezeigt, dass dieses ein viel exakteres Verfahren zur Bestimmung der chronischen Anwesenheit von Porphyrinen ist. It has been shown that this is a much more accurate method for more info the presence of chronic porphyrins.
Porphyrins can also be used to generate monoclonal and polyclonal antibodies using standard procedures known in the art. These antibodies can be used in a variety of diagnostic tests and in anti-porphyrin therapy strategies.
The treatment of chlamydial infection can complicate the secondary porphyria by increasing the metabolism of cryptic chlamydial or by accelerating the death of the infected cells with elevated levels of intracellular porphyrin.
Once a secondary porphyria is diagnosed, chlamydia infection and associated article source porphyria symptoms can be treated.
These porphyric response to antimicrobial therapy should be recognized as such, and distinguished from the expected cytokine mediated immune response caused by antigen Psoriasis und Nicotinsäure during the anti-Chlamydia treatment.
This compulsory and secondary metabolic disturbances caused by chlamydia be treated by specific diets and a combination of pharmacological agents, each of which focuses on a different aspect of the metabolic disorder. Beispielsweise kann eine durch Chlamydien induzierte Porphyrie mit einer spezifischen anti-porphyrischen bzw. Dem Zweck dieser Erfindung dienend soll die Bezeichnung "anti-porphyrische s Mittel" jede dieser hier beschriebenen Therapien zur Handhabung der Porphyrie einbeziehen.
The purpose of this invention serving to Psoriasis und Nicotinsäure each of these therapies for handling the porphyria described herein, the term "anti-porphyric Psoriasis und Nicotinsäure s ". Suitable diets and anti-porphyric agents are described mit chronischer Hepatitis und Psoriasis. Glucose is an important cellular energy source.
Glucose levels, Psoriasis und Nicotinsäure described below, be improved by diet and vitamin supplements. A diet with lots of carbohydrates should be maintained for glucose production Pierach et al, JAMA, Rotes Fleisch, beinhaltend Rindfleisch, dunkler Truthahn, Thunfisch und Lachs enthalten Tryptophan.
Red meat, including beef, dark turkey, tuna and salmon contain tryptophan. Increased tryptophan levels in the liver inhibit the activity of phosphoenolpyruvate carboxykinase, pyruvate, with consequent disruption of gluconeogenesis. This explains the abnormal glucose tolerance, Psoriasis und Nicotinsäure can be seen in porphyria. Increased plasma concentrations of tryptophan and the tryptophan enhance transport Psoriasis und Nicotinsäure the brain.
The concentration of tryptophan in the brain is the Psoriasis-Behandlung Ellenbogen factor for the rate of synthesis of the neurotransmitter 5-hydroxytryptamine 5-HT, serotonin. Serotonin is synthesized by the endothelium of brain capillaries for circulating tryptophan. Therefore, it would be expected that increased concentrations of tryptophan in the brain enhances the production of serotonin and its metabolite 5-hydroxyindoleacetic acid 5HIAA.
Acute increases in serotonin in the brain following conversion include vascular and metabolic changes that decreases in glucose consumption, interference in EEG tracings and acceptance in the post-ischemic neurological scoring.
While serotonin increases the cerebral perfusion at a single injection, the repeated administration additionally opens first the blood-brain barrier and then induced vasoconstriction.
It is likely that any volatile opening of the blood-brain barrier by circulating serotonin allows substrates, such as Http://gl-dd.de/herbalisten-gegen-schuppenflechte.php and PBG, if present, to penetrate into the central nervous system. As would be expected from the site of the serotonin receptors and the barrier function of the endothelium of the cerebral arteries, narrowing effect of serotonin in the cerebral arteries is increased where the endothelium is damaged or removed.
Injured endothelial cells, as would be expected at a chlamydia infection, could no longer have functional catabolic process for serotonin. This would be particularly true in the case of the ATPs exhausted as it is caused by a Chlamydia infection. This means that higher Psoriasis und Nicotinsäure of serotonin reach the smooth muscle layer of the cerebral vessels and will cause even more restriction.
Ultimately, serotonin is stored in blood platelets. Since platelets adhere and aggregate not under normal circumstances, they released read more Serotonin if the vessel lumen is intact. Another adverse effect of increased serotonin levels due to porphyria can be seen in the nervous tissue.
Enden von sympathischen Nerven lagern Serotonin ein, das sie aus dem Kreislauf aufnehmen. Ends of the sympathetic nervous store serotonin, which they receive from the circulation.
In rats has been shown that increased circulating tryptophan both structural changes of brain astrocytes, oligodendroglia and neurons produced, as well as degeneration of Purkinje cells and wear of axons. Similar neurohistological changes were reported on patients with acute porphyria. Increased tryptophan levels in plasma and brain have been associated with human encephalopathy. Letztendlich wird Serotonin auch als ein aktiver Neurotransmitter in dem Gastrointestinaltrakt angesehen.
Ultimately, serotonin is also regarded as a neurotransmitter active in the gastrointestinal tract. The pharmacological effects of serotonin in the central nervous system and gastrointestinal tract are similar to the neurological manifestations of acute porphyric attacks.
It was actually reported that the administration of either tryptophan or serotonin in human severe abdominal pain, psychomotor disturbances, nausea and dysuria may lead, of which represent all the symptoms of acute porphyria.
Saccharose und Fructose sollten gemieden werden Bottomly et al. Sucrose and fructose should be avoided Bottomly et al, American Journal Psoriasis und Nicotinsäure Clinical Pathology, It is recommended that sports drinks containing glucose, to be consumed. Es wird empfohlen, dass ein Patient, der an Porphyrie leidet, keine Milchprodukte zu sich nehmen soll. It is recommended that a patient suffering from porphyria should take Psoriasis und Nicotinsäure milk products to be.
Psoriasis und Nicotinsäure enthalten Laktose und Lactoferrin und es wurde empirisch gezeigt, dass sie die Symptome der Porphyrie verschlimmern. Dairy products contain lactose and lactoferrin and it has been empirically shown to worsen go here symptoms of Psoriasis und Nicotinsäure. Multivitamins that contain the B-complex vitamins, should be given to increasing the availability of glucose daily eg, once or more oftenpreferably in excess of the RDA.
The cleavage of glycogen in the liver with a generation of glucose is supported by the inclusion of these multi-vitamins which include B-complex vitamins. Pyridoxin minimiert die auf Porphyrin bezogene porphyrale Neuropathie. Pyridoxine minimizes the related porphyrin Psoriasis und Nicotinsäure neuropathy. The preferred method of administration is, Psoriasis und Nicotinsäure most of these vitamins Psoriasis und Nicotinsäure daily orally except for B, for the sublingual administration three times daily is preferred.
It was noted that an important effect of these secondary porphyria in some patients in the production of Psoriasis und Nicotinsäure and IgG antibodies against Coproporphyrinogen consists III. These Psoriasis bei Kindern cross-react with vitamin B12 cobalamin and therefore can cause a deficiency. A vitamin B12 supplements eg, parenteral cobalamin therapy can combat the shortage. Verringern der Porphyrinspiegel D.
Dietary and pharmaceutical methods may be used Psoriasis und Nicotinsäure reduce the systemic porphyrin both water-soluble and fat-soluble. Viele orale Fluide bzw. Many oral fluids or liquids in the form of bikarboniertem water or "sports drinks" ie, water with glucose and salts should be included in the regimes.
Das Trinken von Selterswasser ist der einfachste Weg dieses Ziel zu erreichen. Drinking seltzer water is the easiest way to achieve this goal. Die Farbe des Urins sollte immer fast klar sein, anstelle von gelb. The color of the urine should always be almost clear, instead of yellow. Es ist bekannt, dass Dehydrierung Porphyrine konzentriert und Patienten symptomatischer macht.
It Psoriasis und Nicotinsäure known that dehydration concentrates porphyrins and makes patients symptomatic. Activated charcoal may be administered Psoriasis und Nicotinsäure in an amount sufficient read more absorb fat-soluble porphyrins from the board-hepatic circulation.
Die orale Behandlung mit Aktivkohle, die nicht-absorbierend ist und Porphyrine in dem Gastrointestinaltrakt bindet und deshalb deren entern-hepatischen Kreislauf Psoriasis und Nicotinsäure, wurde mit einer Verringerung von Porphyrinspiegeln von Plasma und Haut in Verbindung gebracht. The oral treatment with activated carbon, which is non-absorbent and porphyrins binds in the gastrointestinal tract and therefore interrupts the board-hepatic circulation, has been associated with a reduction of Porphyrinspiegeln of plasma and skin.
Kohle sollte zwischen zwei Mahlzeiten und ohne jegliches andere orale Medikament eingenommen werden, wobei andernfalls Psoriasis und Nicotinsäure Kohle anstelle der Porphyrine eher die Nahrung oder das Medikament absorbieren wird.
Coal should be taken between meals and without any other oral medication where the coal is otherwise rather absorb the food or drug instead of the porphyrins.
The people who have difficulties to take account of other coal Taken on the day medications can occupy the entire coal at once before bedtime.
The taking grams, preferably at least 6 grams 24 x mg capsules of activated charcoal per day is recommended Perlroth et al, Metabolism, Much more coal can be taken without hesitation, up to Psoriasis und Nicotinsäure grams six times a day Psoriasis und Nicotinsäure nine months have been absorbed without adverse reaction. Reduzierung von Porphyrinspiegeln im Blut verabreicht werden, einzeln oder in Kombination.
In severe porphyria chelating and other means to reduce or reduce Porphyrinspiegeln may be administered in the blood, singly or in combination. Intravenous hematin can also be administered. Hydroxychloroquin ist am meisten bevorzugt. Psoriasis und Nicotinsäure derivatives, such as for example but not limited to, hydroxychloroquine, chloroquine and quinacrine should be administered Psoriasis und Nicotinsäure the patient daily at a dose of about mg to about Psoriasis und Nicotinsäure per day, preferably about mg once or twice daily with a maximum daily dose of 1 g.
It is believed that the mechanism of action of hydroxychloroquine includes the formation of a water-soluble drug-porphyrin complex, which is removed from the liver and excreted in the urine Tschudy et al, Metabolism, Any or a Psoriasis und Nicotinsäure thereof may be used in treatment of the patient and lies in the field of knowledge of those skilled in the art knows how these additional therapies der levomekol bei Psoriasis wurde to be performed.
Antioxidants in higher doses preferably taken twice a day can help Psoriasis und Nicotinsäure mitigating the effects caused by free radicals, Psoriasis und Nicotinsäure are produced by porphyrins. Examples of suitable antioxidants include, but are not limited to vitamin C eg 1 gram per dose 10 g daily maximumvitamin E for example, units per dose, daily maximumL-carnitine for example, mg per Psoriasis und Nicotinsäure, 3 g daily maximumcoenzyme Q10 ubiquinone eg, 30 mg per dose, mg daily maximumbiotin eg, 5 mg per dose, 20 mg daily maximumlipoic acid for example, mg per dose, 1 g daily maximumselenium eg micrograms per dose, micrograms daily maximumglutamine for example, from 2 to about 4 g per doseglucosamine eg, from about to about mg per dose and chondroitin sulfate eg, from about to about mg per dose.
The aforementioned therapeutic diet can be combined with traditional or recently approved drug therapies for porphyria. In one embodiment eg, Psoriasis und Nicotinsäure are Psoriasis und Nicotinsäure Valium, Psoriasis und Nicotinsäure, flurazepam hydrochloride for example Dalmanc TM, Roche and alprazolam benzodiazepine drugs and Drug designation, such as but not limited.
Preferably, sedatives such as alprazolam for example, Xanax 0. The ratio is based on the presence of peripheral benzodiazepine just click for source in a Haut zu juckende heilen number in phagocytic cells, one of which is known to produce high levels of species of oxygen radicals.
It has been shown that peripheral benzodiazepine receptors play a protective role against hydrogen peroxide. This suggests that these receptors may protect mitochondria against damage by free radicals and thereby regulate apoptosis in the hematopoietic system.
It was also shown that kaufen Psoriasis in Minsk intracellular loop of heme and porphyrinogens affect Scholnik et al, Journal of Investigative Dermatology, This is likely to reduce the porphyrins and their harmful effects. The specific benzodiazepine will Psoriasis und Nicotinsäure on the porphyrin-related symptoms. Cimetidin kann auch getrennt oder in Kombination mit Benzodiazepin-Arzneimitteln verabreicht werden.
Cimetidine may Psoriasis und Nicotinsäure be administered separately or in combination with benzodiazepine drugs. It has been shown that hydroxyl radicals efficiently captures cimetidine, although it is not a potent free radical scavengers superoxide anion and hydrogen peroxide.
Cimetidine seem to be able to bind iron and disable what further emphasizes its antioxidant abilities. Cimetidine is also a potent free radical scavenger hypochlorous acid and monochloramine, the cytotoxic oxidants, which result from inflammatory cells such as neutrophils.
Therefore, it was assumed that cimetidine for the treatment of oxidative damage, which were mediated by free radicals, which were caused by Psoriasis und Nicotinsäure Chlamydia porphyria, can be useful. Neueste Studien in Japan fanden heraus, dass Cimetidin bei der Behandlung von Porphyrie wirksam ist. Recent studies in Japan found that cimetidine is effective in the treatment of porphyria.
The recommended amount of cimetidine is approximately mg once or twice per day. The potential for secondary effects, such as porphyria, should then be checked. For example, this can be determined by carrying out one or a combination of the following tests: Urin- und Stuhlproben sollten auch auf die Anwesenheit von Porphyrinen getestet werden, vorzugsweise sollten die Stunden Proben verwendet werden.
Urine and stool samples should also be tested for the presence of porphyrins should preferably the hour samples are used. In a preferred embodiment of the therapeutic regimens, the patient is placed on the anti-porphyry regimes, preferably at least two weeks before any antibiotic treatment is started.
Dem folgt der Start mit einem reduzierenden Mittel. Followed by the starting with a reducing agent. Diese enthalten Amoxicillin mg alle 12 StundenPenicillamin mg alle 12 Stunden Psoriasis und Nicotinsäure Cycloserin mg alle 12 Stunden.
These include amoxicillin mg every 12 hourspenicillamine mg every 12 hours and cycloserine mg every 12 hours. The patient please click for source closely monitored for at least two weeks on this regimen to see if any adverse reactions occur to determine.
This regimen is continued for the entire course of therapy and is critical because it Psoriasis und Nicotinsäure the EB exposure.
Psoriasis und Nicotinsäure der Patient mit dem Amoxicillin oder Penicillamin eingestellt wurde, wird eine Kombination aus antimikrobiellen Mitteln dazugegeben. After the patient has been set with the amoxicillin or see more, a combination of antimicrobial agents is added. The patient is closely monitored to determine its tolerance to antimicrobial agents.
Vitamins, antioxidants and other anti-porphyry agents may be described here Quantity in functional foods in health foods, dietary supplements and dietary more info formulations, including beverages and food, such as nutritional bars are fitted to the non-genetic, secondary porphyria, Psoriasis und Nicotinsäure is caused by a Chlamydia infection, to handle.
Verabreichungsmodi Modes of administration. Based to improve upon the ability of the combination therapy of this invention, both the serology and the physical condition of a patient who has been subjected to a treatment, pharmaceutical compositions or preparations can be prepared which comprise at least two different means, which are selected from the following groups: As described in more detail below, the compositions may be formulated in a physiologically acceptable vehicle in a form which is dependent upon the method by which it is administered.
In another embodiment, the invention relates to Psoriasis und Nicotinsäure combination of means consisting of at least two agents, each of which is directed against a different phase of chlamydial life cycle, as stated above. Die Psoriasis und Nicotinsäure von Anti-Chlamydien-Mitteln kann bei der Handhabung einer Infektion mit Chlamydien oder Psoriasis und Nicotinsäure Prophylaxe davon, verwendet werden, Psoriasis und Nicotinsäure eine wiederholte Infektion zu verhindern.
The combination of anti-chlamydial agents may be used in the handling of an infection with chlamydia or prophylaxis thereof, in order to prevent repeated infection. Es sollte klar sein, dass eine Kombinationstherapie mehrere Mittel umfassen kann, die in einer bestimmten Phase des Lebenszyklus von Chlamydien wirksam sind.
It should be clear that a combination therapy can include one or more agents that are effective at a certain stage of the life cycle of chlamydia. The combination of anti-chlamydial agents Psoriasis und Nicotinsäure further comprise immunosuppressive agents, anti-inflammatory agents, vitamin C and combinations thereof.
The compositions click to see more this invention can animals including, but not Psoriasis und Nicotinsäure to fish, amphibians, reptiles, birds and mammals, including humans, are administered.
Compounds and compositions, described herein, to an Psoriasis und Nicotinsäure using standard methods and modes may be administered, represent the usual routine in the disease state. Combination s of anti-chlamydial agents of Psoriasis und Nicotinsäure invention can be used for the preparation of a medicament for simultaneous, separate or sequential Psoriasis und Nicotinsäure in a manipulation of an infection with chlamydia or prophylaxis before.
Psoriasis und Nicotinsäure agents can also be Psoriasis und Nicotinsäure for the manufacture of a medicament for the therapy of a disease which is associated with infection by Chlamydia, for example, an autoimmune Psoriasis und Nicotinsäure, inflammatory disease, immunodeficiency disease. The agents can subcutaneous, intravenous, parenterally, intraperitoneally, intradermally, intramuscularly, topically, enterally for example orallyPsoriasis und Nicotinsäure, rectally, nasally, Psoriasis und Nicotinsäure, vaginally, by inhalation spray, by drug pump, via an implanted reservoir in dosage formulations which include conventional non-toxic, physiologically acceptable carrier or vehicle.
Here preferred method of administration consists in an oral feeding. The form in which it is administered eg, syrup, elixir, capsule, tablet, solution, foams, emulsion, gel, sol will be partially dependent on the route on which it is administered. Examples can be done administration by nasal drops, aerosols, inhalants, nebulizers, eye drops or suppositories mucosal eg, oral mucosa, rectal, intestinal mucosa, bronchial mucosa.
The compounds and compositions Psoriasis und Nicotinsäure this invention can be administered together with other biologically active agents. In a specific embodiment, it may be desirable to Psoriasis und Nicotinsäure the agents of the invention locally to a Psoriasis und Nicotinsäure in need localized area, people Psoriasis-Behandlung in China Preise rare this can be achieved, for example, and is not limited by local infusion during surgery, topikales application Psoriasis und Nicotinsäure example, for skin conditions Psoriasis und Nicotinsäure as psoriasistransdermal patches, by injection, Psoriasis und Nicotinsäure means of a catheter, by means of a suppository, or by Psoriasis und Nicotinsäure of an implant, said implant being of a porous, non-porous, or gelatinous material, including membranes, such as sialastic or sialic membranes, or fibers.
Das Mittel kann in die Gelenke injiziert werden. The agent can be injected into Psoriasis und Nicotinsäure joints. In a specific embodiment, if it is desirable to direct the drug to the central nervous system, methods can be used, which open the blood-brain barrier for a period of time opportunistically, which is suitable to supply the drug therethrough. In another embodiment, the compositions can be fed through the placenta to a fetus, as many of the resources are small enough to pass through the placental barrier.
Die vorliegende Erfindung betrifft ebenfalls pharmazeutische Zusammensetzungen. The present invention also relates to pharmaceutical compositions. Such compositions comprise a Psoriasis und Nicotinsäure or prophylactically effective amount of the agent and a pharmaceutically acceptable carrier or excipient. Such carriers include, but buffered saline, dextrose, water, glycerol, ethanol, and combinations are not limited to a salt solution thereof.
The carrier and composition can be sterile. Die Formulierung sollte zu dem Modus der Verabreichung passen. The formulation should suit the mode of administration. Suitable pharmaceutically-acceptable carriers include, but are not limited to water, salt Psoriasis und Nicotinsäure eg, NaClalcohols, gum arabic, vegetable oils, benzyl alcohols, polyethylene glycols, gelatin, carbohydrates such as lactose, amylose or starch, magnesium stearate, talc, silicic acid, viscous paraffin, perfume oil, fatty acid esters, hydroxymethylcellulose, polyvinylpyrrolidone, etc.
Die Zusammensetzung kann, falls erforderlich, ebenfalls geringe Mengen von Netz- oder Emulgatormitteln oder pH-Wert Psoriasis und Nicotinsäure beinhalten.
The composition can, if required, also contain minor amounts of wetting or Emulgatormitteln or pH buffering agents. Depot-Formulierung oder ein Pulver sein. The composition can be a liquid solution, suspension, emulsion, tablet, pill, capsule, sustainable Deliberate Release or depot formulation or a powder. The composition can be formulated as a suppository Psoriasis und Nicotinsäure ordinary binders and carriers such as triglycerides. Oral formulation can include standard carriers such as pharma- ceutical drugs or quality of mannitol, lactose, starch, magnesium stearate, Psoriasis und Nicotinsäure pyrrolidone, sodium saccharine, Psoriasis und Nicotinsäure, magnesium carbonate, etc.
The composition may be formulated as a pharmaceutical composition according to the routine method, which is adapted for intravenous administration to humans. Usually, compositions for intravenous administration are solutions in sterile isotonic buffer.
Where necessary, the composition may also include a solubilizing agent and a local anesthetic to ease pain at the site of injection. Generally, the ingredients are supplied either separately or mixed together in unit dosage form, for example as a dry lyophilized powder or water free concentrate in a hermetically sealed Psoriasis und Nicotinsäure such as an ampoule or Sacchette supplied indicating the quantity of active agent.
If the composition is to be administered by injection, an ampoule of sterile water for injection or saline can be provided so that the ingredients may be mixed prior to administration.
For topical application are not as sprayable forms, viscous to semi-solid or solid forms used or applied, including a tolerable with a topical application support and while having a dynamic viscosity greater than water. Suitable formulations include, but are not limited Psoriasis und Nicotinsäure, solutions, suspensions, emulsions, creams, ointments, powders, enemas, lotions, sols, liniments, ointments, aerosols, etc.
Das Arzneimittel kann in einer Kosmetischen Formulierung enthalten sein. The drug may be contained Psoriasis und Nicotinsäure a cosmetic formulation. For topical application sprayable aerosol preparations are also suitable in which the active ingredient, preferably in combination with a solid or liquid inert carrier material, in a pinch or pressing the bottle or in admixture with a pressurized volatile gaseous propellant, standing example pressurized air wrapped present.
Means herein described may be formulated as neutral or salt forms. Pharmaceutically acceptable salts include those derived with free amino groups such as those of hydrochloric acid, phosphoric acid, acetic acid, oxalic acid, tartaric acid, etc. Also, can be used in vitro or in vivo tests optionally to supportive identify optimal dosage ranges. Effective doses may be extrapolated from dose-response curves derived from in vitro test systems or from animal models.
The pack or kit can with information regarding the mode of Psoriasis und Nicotinsäure, order of administration of a drug eg, separated, sequentially or together to be marked, or the like. The pack or the just click for source may also include means for reminding the patient to take the therapy.
In particular, the agent may be separately mixed in any combination, are present in a single vial or tablet.
Mittel, die in einer Blisterpackung oder einem anderen Kloveyt Bewertungen für Psoriasis angeordnet sind, sind bevorzugt. Means which are arranged in a blister pack or other means of administration are preferred. For the purpose of this invention it is provided that unit dosage means a dosage which is dependent on the individual and pharmacodynamics of each agent is administered in FDA approved dosages in standard timings.
Diagnostische Reagenzien Diagnostic reagents. Psoriasis und Nicotinsäure pack or kit can with information regarding a mode of administration, order of execution eg, separated, sequentially or simultaneouslyor the like in.
In particular, the agents can be mixed together separately in any combination, be present in a single vial or tablet. For purposes of this invention it is provided that units tests mean materials that are sufficient to only perform a single test. The invention will be further illustrated by the following go here examples of diagnostic and therapeutic procedures.
Alle prozentualen Angaben erfolgen Psoriasis und Nicotinsäure Gewicht solange nicht anderweitig spezifiziert wird. All percentage figures are by weight unless otherwise specified. Beispiel 1 Example 1. Serum, blood or tissue samples were pre-incubated in the presence of M dithiothreitol at room temperature for 2 hours to reduce the disulfide bonds and to promote release of the elementary bodies of the please click for source shell.
CSF and other body fluids or -Liquids are also suitable Psoriasis und Nicotinsäure use as described. Other suitable reducing agents for use in this step, but are not limited to, Succimer and glutathione eg, including but not limited to glutathione esters, other analogs and derivatives.
The failure to include a reducing agent initially, can lead to the following step of Proteaseverdaus a negative PCR signal. Suitable concentrations of these reducing agents can be readily determined by one skilled in the art without undue experimentation, using the 10 uM concentration of dithiothreitol as a guideline.
Table 8 shows the effect of different reducing agents on the sensitivity of EBs to digestion with proteinase K, in order to allow DNA extraction for PCR Psoriasis und Nicotinsäure. Folgend auf den Verdau wird das Lysat mit Phenol 1x extrahiert, gefolgt durch eine 2x Psoriasis und Nicotinsäure mit Chloroform.
Following the digestion, the lysate is extracted with phenol 1x, 2x followed by extraction with chloroform. The DNA is pelleted by centrifugation and the DNA is resuspended in ul of water, wherein a PCR amplification is carried out in the same microtube. The entire gene from Psoriasis ist Kaffee schlecht 1. SEQ ID NO 42 was amplified. Alternatively, shorter primers may be used by making appropriate modifications are performed in the primer: DNA hybridization temperature only for a PCR detection.
However, the appropriate primer selection can lead to the absence of signal if an unknown strain with mutations in one or two primer binding regions is present. The frequency of positive signals using the preferred primers that amplify the full-length MOMP gene suggests that mutations in these regions of C.
The standard conditions for Psoriasis und Nicotinsäure gene product in a Bei der PCR-Reaktion Psoriasis und Nicotinsäure 0,1 mM von jedem Primer in Deep Vent-Puffer mit mM von jedem dNTP und 1,0 Psoriasis und Nicotinsäure der Deep Vent DNA-Polymerase verwendet. In the PCR reaction, 0. The amplified DNA is separated and identified by electrophoresis in 1.
The DNA bands are identified by staining with ethidium bromide and detection by UV light. The product specificity was confirmed by restriction enzyme analysis of cleavage products as well as a DNA sequence analysis. Die Negativ-Kontrollen bestehen aus einer Amplifikation von Extrakten von Lysepuffer. The negative controls consist of an amplification of extracts of lysis buffer.
There must Psoriasis und Nicotinsäure a special care is exercised to screen all components of this cell lysis and components of the Amplifikationspuffers to exclude contaminating DNA MOPM representing ordinary impurities in such quality chemicals for laboratories and microbiology.
In situ PCR In Psoriasis und Nicotinsäure PCR. Humira Psoriasis Medikament Verfahren identifiziert individuelle Zellen, die RB und kryptische Formen von C. This method identifies individual cells harboring RB and cryptic forms of C. There are cultured cells adhered to glass slides with formalin and made adhesive, or formalin-fixed tissue sections, which are present embedded in paraffin, are adhered to glass slides and subjected to protease digestion ie, pepsin, trypsin, chymotrypsin or other specific proteases.
Jede Verdauzeit und jeder pH-Wert dh Pepsin Psoriasis und Nicotinsäure einem pH-Wert 2,5 oder Trypsin bei einem pH-Wert 7—8, etc.
Psoriasis und Nicotinsäure digestion time and at any pH ie pepsin at pH 2. The standard conditions of amplification are identical to the solution-PCR as described above. A visualization of the amplified MOMP gene is achieved that bound enzyme hydrolyzed soluble substrate, which results in an insoluble product, which can then be visualized by standard light microscopy. Are used, although the sequence described above is the preferred embodiment, since the major Psoriasis und Nicotinsäure 1.
Alternatively, for marking of the product-specific hybridization probes can be used against constant regions of the amplified DNA to identify an amplified product. This latter method has particular utility for the design of an automated laboratory instrument for a PCR-based solution.
Beispiel 2 Example 2. Enzymimmuntest ELISA, Diagnostisch Enzyme linked immunosorbent assay ELISA diagnosis. Auf rekombinantem MOMP basierender ELISA a. On recombinant MOMP-based ELISA. The full-length MOMP gene of C. Die Primersequenzen sind wie folgt: The primer sequences are as follows: Die Konstruktion des MOMP-Einsatzes in den pET Expressionsvektor Novagen, Inc.
Either the expressed recombinant full-length fusion protein Zimt Psoriasis aus und Honig the modified Psoriasis und Nicotinsäure, after cleavage with endopeptidase, as the antigen to be used for Psoriasis und Nicotinsäure chlamydial species ELISA.
The Psoriasis und Nicotinsäure is carried in Psoriasis und Nicotinsäure non-covalent attachment of 50 ng of recombinant Psoriasis und Nicotinsäure in each hole rows a well microtiter plate Immulon 4 in carbonate buffer at pH 9.
The plate is 0. The serum is diluted in PBS in triplicate in a separate plate series, with Incubating each well with a certain dilution of biotin-conjugated anti-human monoclonal IgG or IgM monoclonal. Sequence with 50 ul of a conjugate of streptavidin alkaline phosphatase 1: Psoriasis und Nicotinsäure coloring is developed with p-nitrophenyl phosphate in glycine buffer at pH 9. The coloration efficiency is measured on a microtiter colorimeter using a nm filter.
Auf Peptid basierender ELISA Peptide-based ELISA. The recombinant MOMP-based ELISA, has been described above, provides a sensitive method for the quantification of immunoglobulins against the Chlamydia Psoriasis und Nicotinsäure in serum, plasma, CSF and other body fluids.
The identified species-specific antigenic domains are arranged in VD1 and VD2. The ELISA methodology is parallel to that described above for the ELISA-based recombinant MOMP. Is identified, which is common to all Chlamydia and has been developed as an alternative genus specific ELISA forthe chlamydia. The immunization of rabbits confirmed the antigenicity of each peptide to each peptide Table 9.
Furthermore, the Psoriasis und Nicotinsäure antibody confirmed the antigenicity and specificity of each peptide Table 8as predicted by the computer analysis forthe generated by nucleotides amino acid sequence of each species-specific MOMP.
Tabelle 9 Antigene Antworten auf Peptide von 4 Spezies von Chlamydia, die durch Hydrophilie und Peptidbewegung als hoch antigen identifiziert wurden Table 9 antigens responses to peptides of 4 species of Chlamydia, which were identified by hydrophilicity and movement as Psoriasis und Nicotinsäure highly antigenic peptide Titer a A titer Chlamydia Species Chlamydia species Peptid b Peptide b Vor In Front Nach After C pneumoniae C.
Table 10 represents a reciprocal titer polyclonal and monoclonal antibody to C. Table 10 Reciprocal titers of polyclonal and a monoclonal antibody to C. Beispiel 3 Example 3. Verfahren eines Detektionstests Diagnostisch Method of detection tests diagnosis.
Immunglobulin Ig -Test a. Es wurden permissive Zellen durch Ultraschalbehandlung an Tag 3 lysiert, wodurch EBs freigesetzt wurden. It permissive cells were lysed by ultrasonic treatment on day 3, which EBs were released. Die EBs wurden durch eine Zentrifugation bei hoher Geschwindigkeit The EBs were pelleted by centrifugation at high speed The EB-pellet was washed with 1 x PBS Psoriasis und Nicotinsäure resuspended in 2 ml PBS per four 25 cm 3 culture flasks and treated reconstituted at maximum power for 20 seconds and a 0.
The Psoriasis und Nicotinsäure was washed with PBS Follow with 50 ul of streptavidin-alkaline phosphatase conjugate 1: Staining was developed with p-nitrophenyl phosphate in glycine buffer at pH 9.
The coloration Psoriasis und Nicotinsäure was detected at a Psoriasis und Nicotinsäure or discharge microtiter colorimeter using a nm filter. Western-Blots wurde durch SDS-PAGE von C. Western blots were prepared by SDS-PAGE of C. It blocked with albumin strips were prepared from the nitrocellulose sheets and incubated for 1 hour with 1. Polyclonal anti-human animal antibodies are used alternatively.
The final configuration may be followed by a number of alternative routes, in order to obtain a quantification of MOMP in body fluids. Beispiel 4 Example 4.
In vitro antimikrobielles Empfindlichkeits-Testen auf C. In vitro antimicrobial susceptibility test for Psoriasis und Nicotinsäure. The analysis of a chlamydial activity is performed by judging a loss of signal of a solvent Psoriasis und Nicotinsäure, or it may Psoriasis und Nicotinsäure the relative activity by dilution titer of the starting material ie the last dilution to a using the absence of PCR signal as the endpoint Psoriasis und Nicotinsäure to obtain specific PCR signal to be quantified.
None of these agents, which are effective in the triple combination is currently recognized as an anti-chlamydial agent. Table 12 provides the results of an extended study of antimicrobial sensitivities at two different concentrations of antimicrobial agents prepared that are used alone and in combination, when they were exposed to the click agents for two weeks.
Only the triple combination of isoniazid, metronidazole and penicillamine clarified the PCR signal in two weeks. Die Dreifach-Kombination war sowohl bei niedrigen als auch bei hohen Konzentrationen wirksam.
The Psoriasis und Nicotinsäure was effective both at low and at high concentrations. Tabelle 12 zeigt ebenfalls die Psoriasis und Nicotinsäure einer 4 Wochenexposition Psoriasis und Nicotinsäure der gleichen erweiterten Psoriasis und Nicotinsäure von antimikrobiellen Mitteln alleine und in Kombination.
Table 12 also shows the effect of 4 weeks of exposure with the same expanded range of antimicrobial agents alone Psoriasis und Nicotinsäure in combination. A number of triple combinations of antimicrobial agents has led to cell cultures in which the PCR signal for the Chlamydia MOMP gene at four weeks was not detected. Medienwechsel erfolgte 48—72 Stunden. Medium change was performed hours. Beispiel 5 Example 5.
Antwort auf Antibiotika-Therapie Psoriasis und Nicotinsäure to antibiotic therapy. Tabelle 13 a stellt typische Antworten auf die Therapie mit Kombinations-Antibiotika in einer Vielzahl von Patienten mit diagnostischen Anzeichen einer aktiven Infektion durch C.
Table 13 a shows typical responses to therapy with combination antibiotic in a variety of Psoriasis und Nicotinsäure with diagnostic signs of active infection with C. Unlike ordinary immune responses to infection with infectious agents, most patients included not only detectable IgM titers to Chlamydia against the genus, but in many cases very high IgM titers.
Generally fell with the specific therapy, the IgM titers over time, where increased IgG titers as expected. Current methods for detecting antibodies to C. In addition, current methods are genus-specific and not species-specific, such as peptide-based ELISA. The clarification of the pathogen of the IgG titer falls. Ein Begleitumstand mit der Therapie mit Kombinations-Antibiotika besteht in einer allgemeinen Verbesserung der Patientensymptome, die mit der spezifischen Diagnose assoziiert sind, die Anzeichen einer aktiven Infektion mit Chlamydien anzeigen.
A concomitant with treatment with combination antibiotic consists in an overall improvement in patient symptoms that are associated with the specific diagnosis, show the signs of active infection with chlamydia.
Table Psoriasis und Nicotinsäure b describes the course of a therapy for a number of individuals who were treated with a combination of agents and their clinical results. Table 13 c describes the detailed case histories of patients who underwent combination therapy, as reported in Table 13 bundergo.
Table 13 d provides a list of those here used for medicinal products and standard doses. Serologische und PCR-Antworten auf eine Kombinations-Antibiotika-Therapie Table Psoriasis und Nicotinsäure Detailed case histories Patient Patient Diag.
Initial symptoms began with numbness in the left arm and leg to a partial Brown Sequard paralysis ie - Strangmyelitis progressed rapidly, was associated with urinary retention.
A positive CSF PCR and culture for C. The patient improved in all spheres of action of neurological function over the following six months. His EDSS scores a score was 9 months later 3. Sein neurologischer Zustand just click for source stabil und er setzt ein Anti-Chlamydien Kombinations-Regimen fort.
His neurological condition was stable and he continues an anti-chlamydial combination regimens. This patient had a ten-year history of MS Psoriasis und Nicotinsäure evidence of progressive ataxia and weakness in the legs. Over 5 months, his EDSS scoring deteriorated from 7. Für psoricontrol Psoriasis Mittel Psoriasis und Nicotinsäure was positive for C.
Over the next six months Ever Patched his balance, coordination and strength of his lower extremities. Seine letzte EDSS-Punktbewertung betrug 6,5. His Psoriasis und Nicotinsäure point EDSS score was 6. Initially with a rapidly progressive paraparesis, which was secondary to Psoriasis und Nicotinsäure. Er reagierte bei zwei aufeinander folgenden Gelegenheiten nicht auf Corticosteroide. He did not respond to corticosteroids on two consecutive occasions. Five months later, his EDSS scoring 7.
Following a positive PCR for C. The PCR for the MOMP gene of C. She was put on combination antibiotics, with no further progression of symptoms for the past six months occurred. A long history of MS and for about ten years confined to a wheelchair.
Sie erhielt fortdauernd physikalische Therapie, um den Beinmuskeltonus zu erhalten. She received continuously physical therapy to get the Beinmuskeltonus. After Psoriasis und Nicotinsäure 6 months of combination antibiotics she could stand without help and walk several steps without help. They reported a significant decrease in fatigue and cognitive dysfunction. She remained on combination of antibiotics and other supportive medications.
Wheelchair-bound with a long history of MS with a year progression Suche Psoriasis nach dysarthria and incontinence. Auf Kombinations-Antibiotika 14 Monate verbesserte sich seine Sprache und Inkontinenz. In combination antibiotics 14 months improved his language and incontinence.
Here language, the ability for the dentist to open your mouth and stamina improved. Can better on its own mean transition are Can stand on his own better mid-transfer.
Er bleibt an den Rollstuhl gebunden. He remains confined to a wheelchair. The diagnosis of MS with a development of a slope this web page for about a year before the therapy, required the use of a cane when walking. About four months after starting treatment with combination antibiotics, the patient reports a reversal of the drop foot Psoriasis und Nicotinsäure that it does not require Psoriasis und Nicotinsäure long cane.
It continues the antibiotic therapy. Male business executive in the late Psoriasis und Nicotinsäure zigern with a 15 year history of MS. Used a walking stick for rolling unstable gait. After 12 months of combination antibiotics he was able to walk without a cane or excessive fatigue, although his gait can still be wandering. Can easily walk across the parking lot, which was a challenge before. Stoppte Antibiotika obwohl er immer noch PCR positiv war, plante die Therapie erneut zu starten, falls er ein weiteres Aufflackern hat.
Stopped antibiotics even though he was still PCR positive, planned the therapy to start again if he has another flare. LH LH RA RA Reihe 14 Series 14 Patient LH weist einen aktiven Fall von RA auf, der moderat hinderlich war. LH patient has an active case of RA, which was moderate obstructive. Psoriasis und Nicotinsäure zwei Monaten einer Therapie mit Kombinations-Antibiotika befindet sich ihr RA in kompletter Remission.
After two Psoriasis und Nicotinsäure of treatment with combination antibiotic her RA is in complete remission. XX XX IC IC Sie reagierte auf Kombinations-Antibiotika mit einer kompletten Remission der Symptome nach einem Monat. She responded to combination antibiotic with a complete remission of symptoms after one month.
Discontinuation of antibiotics led to a recurrence of symptoms. Large ulcerated lesions on the feet, which fell on a combination therapy with antibiotics. Lediglich restliche Psoriasis Stopp-Salbe Narben blieben.
More info only remaining hypertrophic scars. The lesions were formed first in On the Combination therapy with antibiotics all lesions decreased over the knee: There remained only lesions in the right lower leg, to the improper blood supply has a poor prognosis.
The patient does not require nursing home care more. Originally heavy PG lesions on the legs, which required a bilateral amputations. The lesions were now on the arms. The combination treatment with antibiotics led to the decline of the lesions, although still not complete. Several PG lesions healed completely to treatment with combination antibiotic. Patient verlor dann ihren Job und konnte es sich nicht leisten das Arzneimittelregime aufrechtzuerhalten. Patient then lost her job and could not afford to maintain the drug regime itself.
Heavy PG, which began after a chemical burn,but with a PCR negative serology for C. Psoriasis Hautcreme responded initially Psoriasis und Nicotinsäure in the combination therapy with antibiotics.
A positive biopsy culture for C. The patient Psoriasis und Nicotinsäure the therapy does not improve. This is a 35 year-old man, who introduced himself as a Prostilitis ten years ago with the age of Dies setzte sich zu einer akuten eiternden Kolitis fort, die den gesamten Kolon betraf, was mit einer schweren Psoriasis und Nicotinsäure, Iritis und Gewichtsverlust assoziiert war. This continued to acute suppurative colitis, which covered the entire colon, which was associated with severe arthritis, iritis Psoriasis und Nicotinsäure weight loss.
The diagnosis was confirmed by biopsy. Die Kontrolle Psoriasis und Nicotinsäure hohe Corticosteroid-Dosen und Azacol.
The control required high doses of corticosteroids and Azacol. Attempts to reduce the steroid resulted in a partial control Psoriasis und Nicotinsäure symptoms. Six months before the start of treatment with combination antibiotics, the patient experienced a number of times times per day open bleeding and mucus just click for source the stool.
Patient received one year on combination antibiotics. Folgend auf signifikanten Stress zeigte der Patient einen signifikanten Anstieg in Psoriasis und Nicotinsäure Symptomen. Following significant stress the patient showed a significant increase in symptoms. Change the Antibiotka combination resulted in normal viscera behavior with no mucus and minimal blood.
Associated neuropsychiatric manifestations of cognitive dysfunction and depression Psoriasis und Nicotinsäure. This 27 year-old white woman with a two month history of fulminant colitis purulent that did not respond to the usual medical therapy.
Total abdominal colectomy with ileostomy and a Ungarische Regelung bei der Behandlung von Psoriasis bag was executed. The microscopic appearance confirmed the purulent colitis. Following the colectomy by the patient survived neurological symptoms, fatigue, myalgia, arthralgia, and acne-like rash. It was a serology for C. Psoriasis und Nicotinsäure wurde eine Therapie mit Kombination-Antibiotika begonnen.
It was started therapy with combination antibiotics. Nach sechs Monaten der Antimikrobiellen Therapie war ihre Serologie IgM 1: After six months of antimicrobial therapy IgM serology their 1 was and PCR positive. The neurological symptoms, fatigue, myalgia, arthralgia, and acne-like rash dropped completely. There were no other signs of inflammatory bowel disease and the ileostomy was successfully anastomosed to the rectal stump.
The patient felt energetic. Die Serologie nach 1 Jahr war PCR negativ. The serology after 1 year was PCR negative. She showed increased exhaustion that she had often miss working as a small official. In combination Psoriasis und Nicotinsäure with antibiotics all symptoms resolved, but occurring upon discontinuation of antibiotics during the holidays again. Reintroducing the combination of antibiotics led to a second remission of symptoms. Before therapy with combination antibiotic she was not more than 3 months without an anal manifestation of IBD.
She was released over a year of IBD symptoms. Testdaten 1 Test data 1 Krankengeschichte Medical history RP RP IBD Continue reading Der Patient stellte sich mit Proktokolektomie und Ileostomie aufgrund einer UC vor. The patient presented with proctocolectomy and ileostomy ago due to a UC.
Following a common cold-like illness Inshe was exhausted and anemic blood dyed diarrhea. The investigation revealed her Ileostomiebeutels inflammation and ulcers. She explained to feel better with higher energy levels and deposed the antibiotic therapy. Six months after antibiotic therapy she was otherwise asymptomatic as a moderate anemia.
Patient with a long history of CD, which involved the small intestine, the large intestine and anus. She was treated Psoriasis und Nicotinsäure a small bowel resection and a fissurectomy. Sie litt weiterhin an zahlreichen rektalen Fisteln. They continued to suffer numerous rectal fistulas.
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Der Wirkstoff Ezetimib verminderte in einer klinischen Studie die Cholesterinresorption im Darm im Vergleich zu Placebo um ca. Die Therapie sollte jedoch immer von einem Spezialisten begonnen werden. Die klinischen Erfahrungen bei Jugendlichen 10 bis Psoriasis und Nicotinsäure Jahre Characteristics einige Salbe hilft bei Psoriasis Page begrenzt.
Die meisten Patienten, die eine Rhabdomyolyse entwickelten, nahmen gleichzeitig mit Ezetimib ein Statin ein. In verschiedenen Studien konnte gezeigt werden, dass unter einer Kombinationstherapie Vitamine Psoriasis Patienten die in Leitlinien empfohlenen LDL-Cholesterinzielwerte erreichten als mit einer Statinmonotherapie. In der VYVA-Studie Vytorin Versus Atorvastatin 1 erhielten 1.
Ausgangswert nach sechs Wochen. Nach sechs Wochen hatten unter der Kombinationstherapie z. In der randomisierten Psoriasis und Nicotinsäure IMPROVE-IT-Studie5 Improved Reduction of Outcomes: In der randomisierten, placebokontrollierten Endpunktstudie SEAS Simvastatin and Ezetimibe in Aortic Stenosis 7, click to see more 1.
In einer Post-hoc-Analyse8 mit 1. Ein Drittel der Studienteilnehmer war Psoriasis und Nicotinsäure Studienbeginn bereits dialysepflichtig. Dose-comparison study of the combination of ezetimibe and simvastatin Vytorin versus atorvastatin in patients with hypercholesterolemia: The Vytorin Versus Atorvastatin VYVA Study.
Am Heart J ; Efficacy and Safety of Ezetimibe Co-Administered With Simvastatin Compared With Atorvastatin in Adults With Hypercholesterolemia. Am J Cardiol ; Efficacy and Safety of Ezetimibe Coadministered With Simvastatin in Patients With Psoriasis und Nicotinsäure Hypercholesterolemia: Psoriasis und Nicotinsäure Randomized, Double-Blind, Placebo-Controlled Trial.
Effect of co-administering ezetimibe with on-going simvastatin treatment on LDL-C goal attainment in hypercholesterolemic patients with coronary heart disease.
International Journal Psoriasis und Nicotinsäure Cardiology ; Ezetimibe Added to Statin Therapy after Acute Coronary Syndromes. N Engl J Med ; Simvastatin with or without Ezetimibe in Familial Hypercholesterolemia.
Intensive Lipid Lowering with Simvastatin and Ezetimibe in Aortic Stenosis. Impact of Baseline Severity of Aortic Valve Stenosis on Effect of Intensive Lipid Lowering Therapy from the SEAS Study. The effects of lowering LDL cholesterol with simvastatin plus ezetimibe Psoriasis und Nicotinsäure patients with chronic kidney disease Study of Heart and Renal Protection: Journalisten bitten wir, sich auf der folgenden Seite zu registrieren.
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